Polypeptides with endoglucanase activity and uses thereof
AssigneeDanisco US Inc.
InventorsRichard Bott, David A. Estell, Neeraj Pandey, Sina Pricelius, Jian Yao
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Abstract
Disclosed herein are cellulase variants, or active fragments thereof, and polynucleotides encoding same, where the cellulase variants, or active fragments thereof, have endoglucanase activity. Also disclosed herein are compositions comprising the cellulase variants, or active fragments thereof; vectors and/or host cells comprising the polynucleotides encoding the cellulase variants, or active fragments thereof; and methods for making and/or using the cellulase variants, or active fragments thereof and/or compositions containing same; where the cellulase variants, or active fragments thereof, have endoglucanase activity.
Claims21
A cellulase variant, comprising an amino acid sequence comprising two or more mutations at two or more positions corresponding to SEQ ID NO:1
- wherein said two or more mutations comprise a threonine at position 36 and at least one additional mutation at a position selected from 4, 23, 29, 32, 34, 44, 51, 65, 77, 80, 87, 90, 97, 98, 99, 102,112, 116, 119, 135, 136, 142, 153, 154, 156, 157, 161, 163, 178, 192, 194, 202, 204, 205, 206, 208, 210, 212, 217, 221, 222, 225, 227, 232, 233, 236, 237, 238, 241, 247, 249, and 258,
- wherein said variant has endoglucanase activity, wherein said variant has an amino acid sequence having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1, and wherein the amino acid positions of the variant, are numbered by correspondence with the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.