Cocaine esterase mutant and use thereof
AssigneeHangzhou Normal University
InventorsXiabin Chen, Jianzhuang Yao, Shurong Hou, Xingyu Deng, Yun Zhang, Junsen Tong
Life of this patent
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Abstract
Disclosed are a cocaine esterase mutant and use thereof. The cocaine esterase mutant is obtained by mutating a wildtype cocaine esterase, an amino acid sequence of the wildtype cocaine esterase is shown as SEQ ID No. 1, the cocaine esterase mutant is T172R/G173Q/L196C/I301C, or additionally added with V116K point mutation, or additionally added with A51 site mutation, and the A51 site mutation is L, Y, V, F or W. Catalytic efficiency of the cocaine esterase mutant screened on a cocaine toxic metabolite benzoylecgonine is greatly improved compared with that of a wildtype enzyme.
Claims4
A cocaine esterase mutant obtained by mutating a wildtype cocaine esterase, wherein an amino acid sequence of the wildtype cocaine esterase is shown as SEQ ID NO:
- 1, and
- the cocaine esterase mutant is one of the following:
- (1) the SEQ ID NO:
- 1 having a mutation at a site of the 116th position, wherein the mutation is V116K,
- (2) the SEQ ID NO:
- 1 having three mutations at sites of the 172nd position, the 173rd position, and the 116th position, wherein the three mutations are:
- T172R, G173Q, and V116K,
- (3) the SEQ ID NO:
- 1 having five mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, wherein the five mutations are:
- T172R, G173Q, L196C, I301C, and V116K,
- (4) the SEQ ID NO:
- 1 having six mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, and 51st position, wherein the six mutations are:
- T172R, G173Q, L196C, I301C, V116K, and A51L,
- (5) the SEQ ID NO:
- 1 having six mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, and 51st position, wherein the six mutations are:
- T172R, G173Q, L196C, I301C, V116K, and A51Y,
- (6) the SEQ ID NO:
- 1 having six mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, and 51st position, wherein the six mutations are:
- T172R, G173Q, L196C, I301C, V116K, and A51V,
- (7) the SEQ ID NO:
- 1 having six mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, and 51st position, wherein the six mutations are:
- T172R, G173Q, L196C, I301C, V116K, and A51F, or
- (8) the SEQ ID NO:
- 1 having six mutations at sites of the 172nd position, the 173rd position, the 196th position, the 301st position, the 116th position, and 51st position, wherein the six mutations are:
- T172R, G173Q, L196C, I301C, V116K, and A51W.
Dependencies are inferred from the claim text.