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US 12,180,515 B2Granted
Omega-hydroxylase-related fusion polypeptide variants with improved properties
AssigneeGenomatica, Inc.
InventorsAndreas Schirmer, Baolong Zhu, Emanuela Popova
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Abstract
The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.
Claims20
A cytochrome P450 CYP153A reductase (CYP153A-reductase) hybrid fusion polypeptide variant comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, wherein:
- the CYP153A-reductase hybrid fusion polypeptide variant comprises at least one mutation at one or more amino acid positions corresponding to positions 77, 159, 197, 451, or 643 of SEQ ID NO:6;
- and
- the CYP153A-reductase hybrid fusion polypeptide variant catalyzes the conversion of a fatty acid or derivative thereof to an ω-hydroxy fatty acid or derivative thereof.
Dependencies are inferred from the claim text.