Modified DAAO enzyme and application thereof
AssigneeHunan Lier Biotech Co., Ltd
InventorsXinkai Xie, Wei Xu, Junying Fan
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Abstract
The present invention refers to a modified D-amino acid oxidase (DAAO). In particular, the modified DAAO of the present invention has the activity of catalyzing the oxidation of D-glufosinate into PPO. Further, the modified DAAO of the present invention has increased activity of catalyzing the oxidation of D-glufosinate into PPO and/or increased stability as compared to SEQ ID NO: 4. The present invention also refers to the polynucleotide encoding the modified DAAO of the present invention, the vector and host cell expressing the modified DAAO of the present invention, and the method of producing L-glufosinate with the modified DAAO and host cell of the present invention.
Claims13
A modified D-amino acid oxidase (DAAO) comprising amino acid substitutions at positions 54, 56, 58 and 213 as compared to its wild-type DAAO, wherein the positions are numbered by reference to SEQ ID NO:
- 2, wherein the amino acid at position 54 is substituted by I, V, T or L, the amino acid at position 56 is substituted by N, the amino acid at position 58 is substituted by H or Q, and the amino acid at position 213 is substituted by S or T, and wherein the modified DAAO has the activity of catalyzing the oxidation of D-glufosinate into PPO.
Dependencies are inferred from the claim text.