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US 12,180,508 B2Granted
Method of preparing in vitro-matured human intestinal organoids and use thereof
AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsMi Young Son, Janghwan Kim, Soo Jin Oh, Cho Rok Jung, Hyun Soo Cho, Hana Lee, Kwang Bo Jung
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Abstract
Provided are a method of preparing in vitro-matured intestinal organoids, and intestinal organoids prepared by the method.
Claims14
A method of preparing an in vitro-matured human intestinal organoid, the method comprising
- preparing an immature intestinal organoid by differentiating stem cells in a first medium, and
- culturing the immature intestinal organoid in a second medium containing T-lymphocytes, Interleukin 2 (IL-2), or a combination thereof, wherein the first medium and the second medium are different.
A method of maturing a human intestinal organoid in vitro, the method comprising
- culturing an immature intestinal organoid obtained by differentiating stem cells in a medium containing Interleukin 2 (IL-2),
- wherein the intestinal organoid is not co-cultured with immune cells.
A method of producing a functional human intestinal organoid, comprising
- culturing an immature intestinal organoid obtained by differentiating stem cells, in a medium containing T-lymphocytes, Interleukin-2, or a combination thereof,
- wherein the resulting functional intestinal organoid comprises mature functional goblet cells and/or mature functional enteroendocrine cells.
Dependencies are inferred from the claim text.