# US 12,180,505 B2 · Nanobarcode for controlling cell adhesion and differentiation of stem cells > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,180,505 B2 - Document ID: US12180505B2 - Status: Granted - Kind: B2 - Application number: 17/246,376 - Filed: 2021-04-30 - Published: 2021-11-25 - Granted: 2024-12-31 - Earliest claimed: 2020-05-22 - Assignees: Korea University Research and Business Foundation - Applicants: Korea University Research and Business Foundation - Inventors: Heemin Kang; Young Keun Kim; Sunhong Min; Yoo Sang Jeon; Hyojun Choi - Web page: https://patentgrep.com/patent/US12180505B2 ## CPC - A61L 27/18: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds - C12N 5/0662: {Stem cells} - C07K 5/0817: {the first amino acid being Arg} - B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery - B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites - C12N 2501/998: Proteins not provided for elsewhere - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES ## Abstract Disclosed are a nanobarcode for controlling adhesion and differentiation of stem cells and a method of controlling adhesion and differentiation of stem cells by using nanobarcodes. The method of controlling adhesion and differentiation of stem cells of the present invention may efficiently control adhesion and differentiation of stem cells in vivo or in vitro by tuning periodicity and sequences of a ligand peptide (RGD) of a nanobarcode. ## Claims ### Claim 1 A nanobarcode for controlling adhesion and differentiation of stem cells, the nanobarcode comprising: a nanobarcode in which a first segment including iron (Fe) and a second segment including gold (Au) are repeatedly formed; and an integrin ligand peptide bound to the second segment of the nanobarcode, wherein the integrin ligand peptide comprises a uniform distribution in a monolayer, and the nanobarcode comprises a density of 0.0235 to 0.0277 per square micrometer (μm2), and the stem cells adhere to the second segment. ### Claim 2 (depends on 1) The nanobarcode of claim 1, wherein the nanobarcode is provided in a rod shape satisfying Equation 1 or Equation 2 below, [L(M1M2)q] [Equation 1] [L(M1M2M2M1)q] [Equation 2] herein, M1 is the first segment, M2 is the second segment, q is the number of times of the repetition of the first and second segments, and L is lengths of the first and second segments. ### Claim 3 (depends on 2) The nanobarcode of claim 2, wherein Equations 1 and 2 are any one of [30(M1M2)10], [75(M1M2)4], [75(M1M2M2M1)2], [150(M1M2)2], [150(M1M2M2M1)1], and [300(M1M2)1]. ### Claim 4 (depends on 1) The nanobarcode of claim 1, wherein each of the first segment and the second segment is provided in a rod shape, and a length of the first segment is the same as a length of the second segment. ### Claim 5 (depends on 1) The nanobarcode of claim 1, wherein the first segment has a structure in which a carboxylate is substituted. ### Claim 6 (depends on 1) The nanobarcode of claim 1, wherein the nanobarcode has a rod shape having a circular cross section with a diameter of 50 nm to 100 nm and a length of 200 to 1,000 nm. ### Claim 7 (depends on 1) The nanobarcode of claim 1, wherein the integrin ligand peptide includes a thiolated integrin ligand peptide, and has a structure in which a thiol group of the integrin ligand peptide is chemically bound to the second segment.