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US 12,180,502 B2Granted
Nucleic acid-guided nucleases
AssigneeInscripta, Inc.
InventorsAndrew Garst, Ryan T. Gill, Tanya Elizabeth Warnecke Lipscomb
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Abstract
Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
Claims22
A nucleic acid-guided nuclease system comprising:
- (a) a nucleic acid-guided nuclease comprising at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
- 7 and one or more nuclear localization sequences, or a nucleic acid molecule encoding the nucleic acid-guided nuclease and the one or more nuclear localization sequences;
- and
- (b) an engineered guide nucleic acid, or a nucleic acid molecule encoding the engineered guide nucleic acid, wherein the engineered nucleic acid comprises a region for complexing with the nucleic acid-guided nuclease and a region for hybridizing with a target sequence, thereby directing the nucleic acid-guided nuclease to the target sequence,
- wherein the region for complexing with the nucleic acid-guided nuclease is a pseudoknot region.
2 more elements
Dependencies are inferred from the claim text.