# US 12,180,453 B2 · Systems, devices, and methods for electroporation within a cell processing system > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,180,453 B2 - Document ID: US12180453B2 - Status: Granted - Kind: B2 - Application number: 18/611,632 - Filed: 2024-03-20 - Published: 2024-09-26 - Granted: 2024-12-31 - Earliest claimed: 2023-03-21 - Assignees: Cellares Corporation - Applicants: Cellares Corporation - Inventors: Chihwei Chang; Wayne Grout - Web page: https://patentgrep.com/patent/US12180453B2 ## CPC - C12M 35/02: {Electrical or electromagnetic means, e.g. for electroporation or for cell fusion} - C12M 23/22: {Transparent or translucent parts (glassware for laboratory use B01L3/00)} - C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)} - C12N 15/87: Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The present disclosure relates to systems, devices, and methods for electroporating. In an embodiment, the present disclosure relates to an electroporation system, comprising at least one electroporation chamber, each electroporation chamber comprising a first electrode and a second electrode, a plurality of ports comprising an inlet port at a first portion of each electroporation chamber, and an outlet port at a second portion of each electroporation chamber, a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port and the outlet port, and an automated system for electroporation operatively connected to the first electrode and the second electrode of the at least one electroporation chamber. ## Claims ### Claim 1 An electroporation chamber, comprising: a first electrode and a second electrode; a plurality of ports comprising an inlet port at a first portion of the electroporation chamber, and an outlet port at a second portion of the electroporation chamber; a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port, the outlet port, and each of a plurality of ports via auxiliary channels extending therefrom; and at least one window disposed on a wall of the electroporation chamber and providing a view to a segment of the fluid conduit, wherein the at least one window comprises a first window and a second window, the first window having a view to a first segment of the fluid conduit and the second window having a view to a second segment of the fluid conduit, and wherein the second window is proximate a first auxiliary channel of the plurality of auxiliary channels that is in fluid communication with the outlet port. ### Claim 2 (depends on 1) The electroporation chamber of claim 1, wherein the plurality of ports further comprises a vent port in fluid communication with the fluid conduit and arranged at the first portion of the electroporation chamber. ### Claim 3 (depends on 1) The electroporation chamber of claim 1, wherein the fluid conduit has a volume defined by a distance between the first electrode and the second electrode. ### Claim 4 (depends on 3) The electroporation chamber of claim 3, wherein the volume of the fluid conduit is between about 0.5 μL to about 2.5 mL. ### Claim 5 (depends on 1) The electroporation chamber of claim 1, wherein a distance between the first electrode and the second electrode is between about 0.5 mm and about 5 mm. ### Claim 6 (depends on 1) The electroporation chamber of claim 1, further comprising a first housing and a second housing, wherein the first electrode, the fluid conduit, and the second electrode are positioned between the first housing and the second housing. ### Claim 7 (depends on 1) The electroporation chamber of claim 1, wherein the segment of the fluid conduit is proximate the first portion of the electroporation chamber. ### Claim 8 (depends on 1) The electroporation chamber of claim 1, wherein the segment of the fluid conduit is proximate the second portion of the electroporation chamber. ### Claim 9 (depends on 1) The electroporation chamber of claim 1, wherein the at least one window comprises a first window and a second window, the first window having a view to a first segment of the fluid conduit and the second window having a view to a second segment of the fluid conduit. ### Claim 10 (depends on 2) The electroporation chamber of claim 2, wherein the fluid conduit is in fluid communication with each of the plurality of ports via auxiliary channels extending therefrom. ### Claim 11 (depends on 10) The electroporation chamber of claim 10, wherein each of the auxiliary channels is fluidically coupled the fluid conduit and a respective one of the plurality of ports. ### Claim 12 (depends on 11) The electroporation chamber of claim 11, wherein an auxiliary channel of the auxiliary channels fluidically coupling the fluid conduit to the outlet port is curvilinear. ### Claim 13 (depends on 11) The electroporation chamber of claim 11, wherein an auxiliary channel in fluid communication with the outlet port is curvilinear, and wherein the second window is proximate the curvilinear auxiliary channel. ### Claim 14 (depends on 13) The electroporation chamber of claim 13, wherein the second window is proximate a base of the curvilinear auxiliary channel. ### Claim 15 (depends on 11) The electroporation chamber of claim 11, wherein the first window is proximate the auxiliary channel in fluid communication with the vent port. ### Claim 16 (depends on 15) The electroporation chamber of claim 15, wherein the first window is proximate a base of the auxiliary channel in fluid communication with the vent port. ### Claim 17 (depends on 2) The electroporation chamber of claim 2, wherein the inlet port arranged at the first portion of the electroporation chamber is further arranged toward a first side wall of the electroporation chamber, and wherein the vent port arranged at the first portion of the electroporation chamber is further arranged toward a second side wall of the electroporation chamber. ### Claim 18 (depends on 4) The electroporation chamber of claim 4, wherein, along an axis defined from the first portion of the electroporation chamber to the second portion of the electroporation chamber, the fluid conduit has a triangular cross-section. ### Claim 19 (depends on 1) The electroporation chamber of claim 1, wherein each of the plurality of ports extends through the first electrode and/or the second electrode. ### Claim 20 An electroporation chamber, comprising: a first electrode and a second electrode; a plurality of ports comprising an inlet port at a first portion of the electroporation chamber and arranged toward a first side wall of the electroporation chamber, an outlet port at a second portion of the electroporation chamber; and a vent port at the first portion of the electroporation chamber and arranged toward a second side wall of the electroporation chamber; a fluid conduit arranged between the first electrode and the second electrode, the fluid conduit being in fluid communication with the inlet port, the outlet port, and the vent port; and at least one window disposed on a wall of the electroporation chamber and providing a view to a segment of the fluid conduit. ### Claim 21 (depends on 20) The electroporation chamber of claim 20, wherein the fluid conduit has a volume defined by a distance between the first electrode and the second electrode. ### Claim 22 (depends on 21) The electroporation chamber of claim 21, wherein the volume of the fluid conduit is between about 0.5 μL to about 2.5 mL. ### Claim 23 (depends on 20) The electroporation chamber of claim 20, wherein a distance between the first electrode and the second electrode is between about 0.5 mm and about 5 mm. ### Claim 24 (depends on 20) The electroporation chamber of claim 20, further comprising a first housing and a second housing, wherein the first electrode, the fluid conduit, and the second electrode are positioned between the first housing and the second housing. ### Claim 25 (depends on 20) The electroporation chamber of claim 20, wherein the segment of the fluid conduit is proximate the first portion of the electroporation chamber. ### Claim 26 (depends on 20) The electroporation chamber of claim 20, wherein the segment of the fluid conduit is proximate the second portion of the electroporation chamber. ### Claim 27 (depends on 20) The electroporation chamber of claim 20, wherein the at least one window comprises a first window and a second window, the first window having a view to a first segment of the fluid conduit and the second window having a view to a second segment of the fluid conduit.