# US 12,180,345 B2 · Method for improving triboelectric output performance of protein film by changing protein structure

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- Number: US 12,180,345 B2
- Document ID: US12180345B2
- Status: Granted
- Kind: B2
- Application number: 17/775,894
- Filed: 2020-10-29
- Published: 2022-12-01
- Granted: 2024-12-31
- Earliest claimed: 2020-08-25
- Assignees: Zhejiang University
- Applicants: Zhejiang University
- Inventors: Jianfeng Ping; Chengmei Jiang; Yibin Ying
- Web page: https://patentgrep.com/patent/US12180345B2

## CPC

- C08J 5/18: Manufacture of films or sheets
- C07K 1/02: in solution {(C07K1/003, C07K1/006 take precedence)}
- C07K 1/13: Labelling of peptides
- C07K 14/415: from plants
- H02N 1/04: Friction generators
- C08J 2389/00: Characterised by the use of proteins; Derivatives thereof
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- H02N: ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR

## Abstract

The invention discloses a method for improving triboelectric output performance of a protein film by changing a protein structure. Indissolvable protein powder and a trace amount of another protein powder are co-dissolved in a strong alkaline aqueous solution and maintained for a period of time, and then acidifying treatment is performed to achieve neutral condition to allow charge redistribution to induce refolding of the protein, which results in burying of hydrophobic groups of the protein and exposure of charged groups. Therefore, the solubility of the protein is remarkably improved, and a uniform protein solution is formed under a neutral condition. The plant protein structure is changed through a pH cycle process, a surface group exposure condition is adjusted, and the output performance of the plant protein film is greatly improved.

## Claims

### Claim 1

A method for improving a triboelectric output performance of a protein film by changing a protein structure, comprising pretreating a protein powder to obtain a protein solution, adding a plasticizer, and denaturing the protein through a heat treatment to obtain a more extensive structure required for film forming; evaporating a solvent and drying to obtain a transparent and flexible protein film with a uniform texture; wherein pretreating the protein powder to obtain the protein solution comprises co-dissolving a first protein powder and a minor amount of another protein powder in an alkaline aqueous solution and maintaining for a period of time, and performing an acidifying treatment to achieve neutral condition to form the protein solution under a neutral condition,
wherein the first protein powder is a plant protein comprising rice protein, peanut protein isolate, soybean protein isolate, wheat glutelin, or zein, and
wherein the alkaline aqueous solution is a sodium hydroxide solution to adjust pH to a range between 11 and 13, and is maintained for a period of time for 2 to 8 hours.

### Claim 2 (depends on 1)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 1, wherein the another protein powder is a protein powder with a protein structure different from the first protein powder, and a mass ratio between the first protein powder and the another protein powder is 1:0.005 to 1:0.1.

### Claim 3 (depends on 1)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 1, wherein the acidifying treatment adopts a step of adding hydrochloric acid and adjusting pH to a range between 6 and 8.

### Claim 4 (depends on 1)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 1, wherein the plasticizer is polyol, and an amount of addition of the plasticizer is 20 to 50% (w/w) of the protein powder.

### Claim 5 (depends on 1)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 1, wherein the process of obtaining the protein solution from the pretreatment is carried out under a stirring condition.

### Claim 6 (depends on 1)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 1, wherein the protein film serves as an electron-donating layer of a triboelectric nanogenerator, which is stacked face-to-face with a triboelectric negative layer, respective rear surfaces of the protein film and the triboelectric negative layer are adhered or plated with electrodes, or only the rear surface of the protein film or the rear surface of the triboelectric negative layer is provided with a grounded electrode, thereby fabricating the triboelectric nanogenerator.

### Claim 7 (depends on 6)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 6, wherein the triboelectric negative layer comprises polytetrafluoroethylene (Teflon), polydimethylsiloxane (PDMS), polyvinyl chloride (PVC), polyimide (Kapton), silicon rubber (Ecoflex), or polylactic acid (PLA).

### Claim 8 (depends on 6)

The method for improving the triboelectric output performance of the protein film by changing the protein structure according to claim 6, wherein the triboelectric nanogenerator has four operating modes, comprising a vertical contact-separation mode, a lateral-sliding mode, a single-electrode mode and a freestanding triboelectric-layer mode.