# US 12,180,245 B1 · Separation and identification of myricetin-3-ogalactoside from casuarina glauca

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- Number: US 12,180,245 B1
- Document ID: US12180245B1
- Status: Granted
- Kind: B1
- Application number: 18/395,180
- Filed: 2023-12-22
- Granted: 2024-12-31
- Earliest claimed: 2023-12-22
- Assignees: King Faisal University
- Applicants: King Faisal University
- Inventors: Maged Elsayed Mohamed; Nancy Safwat Younis; Bandar Essa Eldubiab; Azza M. El Shafae; Nora Tawfeek; Eman Fikry
- Web page: https://patentgrep.com/patent/US12180245B1

## CPC

- C07H 1/08: from natural products
- A61K 36/185: Magnoliopsida (dicotyledons)
- C07H 17/07: Benzo[b]pyran-4-ones
- A61K 2236/15: involving mechanical treatment, e.g. chopping up, cutting or grinding
- A61K 2236/333: using mixed solvents, e.g. 70% EtOH
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The disclosure of the present patent application relates generally to the separation and identification of myricetin-3-O-galactoside from Casuarina glauca Sieber branchlets.

## Claims

### Claim 1

A process for separation and identification of myricetin-3-O-galactoside comprising:
providing fresh branchlets of a Casuarina glauca plant;
shade drying and powdering the fresh branchlets to obtain a Casuarina glauca plant powder;
soaking 300 g of the Casuarina glauca plant powder with 70% ethyl alcohol to obtain a Casuarina glauca plant extract;
concentrating the Casuarina glauca plant extract to obtain a residue;
dissolving the residue to obtain an aqueous mixture;
mixing and sonicating the aqueous mixture;
centrifuging the aqueous mixture to obtain a supernatant;
separating myricetin-3-O-galactoside from the supernatant; and
wherein the soaking step is repeated three times in successive portions of 1 L of the 70% ethyl alcohol.

### Claim 2 (depends on 1)

The process of claim 1, wherein the plant is Casuarina glauca Sieber.

### Claim 3 (depends on 1)

The process of claim 1, wherein the supernatant is about 1 ml of a mobile phase solution comprising water:methanol:acetonitrile (50:25:25).

### Claim 4 (depends on 1)

The process of claim 1, wherein the mixing and sonicating step comprises:
vortexing the aqueous mixture for about 2 minutes; and
subjecting the aqueous mixture to ultrasonication for about 10 minutes.