# US 12,179,208 B2 · Flow cell receiver and methods of use

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- Number: US 12,179,208 B2
- Document ID: US12179208B2
- Status: Granted
- Kind: B2
- Application number: 18/483,259
- Filed: 2023-10-09
- Published: 2024-02-22
- Granted: 2024-12-31
- Earliest claimed: 2019-12-23
- Assignees: Singular Genomics Systems, Inc.
- Applicants: Singular Genomics Systems, Inc.
- Inventors: Sandor Kovacs; David Baranson; Eli N. Glezer
- Web page: https://patentgrep.com/patent/US12179208B2

## CPC

- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 2200/025: Align devices or objects to ensure defined positions relative to each other
- B01L 2200/027: for microfluidic devices
- B01L 2200/0689: Sealing
- B01L 2300/168: Specific optical properties, e.g. reflective coatings
- B01L 2300/18: Means for temperature control
- B01L 2300/1822: using Peltier elements
- B01L 2300/1844: using fans
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.

## Claims

### Claim 1

A method of detecting a fluorescently labeled nucleotide in a sample using a receiving device comprising a platform, a vacuum port and a magnet, wherein the vacuum port and the magnet are configured to align, secure, and retain a solid support on the platform, said method comprising:
placing a solid support comprising a sample on the platform, wherein the sample comprises the fluorescently labeled nucleotide;
directing an excitation beam onto the sample; and
detecting the fluorescently labeled nucleotide in the sample by detecting a light emission from the sample.

### Claim 2 (depends on 1)

The method of claim 1, wherein the receiving device further comprises a plurality of vacuum ports.

### Claim 3 (depends on 1)

The method of claim 1, wherein the vacuum port is configured to provide sufficient vacuum pressure to ensure a maximum physical contact between the solid support and the platform.

### Claim 4 (depends on 1)

The method of claim 1, wherein the receiving device further comprises a fluidic system configured to provide one or more fluids to the solid support.

### Claim 5 (depends on 1)

The method of claim 1, further comprising providing a constructive interference with the magnet by orienting the magnet.

### Claim 6 (depends on 1)

The method of claim 1, wherein the vacuum port and the magnet prevents a significant movement of the solid support.

### Claim 7 (depends on 1)

The method of claim 1, wherein the platform further comprises a visible light absorbing coating, or an ultraviolet light absorbing coating, or an infrared light absorbing coating, or a combination thereof.

### Claim 8 (depends on 1)

The method of claim 1, wherein the receiving device further comprises a thermoelectric heating element configured to modulate temperature of the platform.

### Claim 9 (depends on 8)

The method of claim 8, wherein the thermoelectric heating element is a Peltier device.

### Claim 10 (depends on 3)

The method of claim 3, wherein the sufficient vacuum pressure is less than about 760 torr.

### Claim 11 (depends on 1)

The method of claim 1, wherein said receiving device further comprises a circuit board.

### Claim 12 (depends on 11)

The method of claim 11, wherein the solid support further comprises an electronically erasable programmable read only memory chip, and the circuit board is configured to contact to the electronically erasable programmable read only memory chip.

### Claim 13 (depends on 11)

The method of claim 11, wherein said circuit board is configured to store information and to process the information, and/or to modulate a radio-frequency signal and demodulate the radio-frequency signal.

### Claim 14 (depends on 1)

The method of claim 1, wherein the fluorescently labeled nucleotide comprises a 3′ reversible terminator.

### Claim 15 (depends on 1)

The method of claim 1, wherein the fluorescently labeled nucleotide forms part of a nucleic acid molecule.

### Claim 16 (depends on 15)

The method of claim 15, wherein the nucleic acid molecule is hybridized to a target polynucleotide.

### Claim 17 (depends on 1)

The method of claim 1, wherein the sample further comprises a plurality of fluorescently labeled nucleotides.

### Claim 18 (depends on 17)

The method of claim 17, further comprising adjusting a position of the solid support using the platform, and detecting a second fluorescently labeled nucleotide in the sample.

### Claim 19 (depends on 1)

The method of claim 1, wherein the sample comprises four fluorescently labeled nucleotides, wherein each of the four fluorescently labeled nucleotides comprises a different fluorescent label.

### Claim 20 (depends on 1)

The method of claim 1, wherein the receiving device further comprises an input port and an output port.