# US 12,179,205 B2 · Reagent packaging devices and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,179,205 B2 - Document ID: US12179205B2 - Status: Granted - Kind: B2 - Application number: 17/808,187 - Filed: 2022-06-22 - Published: 2022-10-06 - Granted: 2024-12-31 - Earliest claimed: 2017-05-11 - Assignees: CytoChip Inc. - Applicants: CytoChip Inc. - Inventors: Wendian Shi; Yuzhe Ding - Web page: https://patentgrep.com/patent/US12179205B2 ## CPC - B01L 3/52: {Containers specially adapted for storing or dispensing a reagent (B01L3/02 takes precedence; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002)} - B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces} - B32B 3/20: of hollow pieces, e.g. tubes; of pieces with channels or cavities - B32B 3/266: {characterised by an apertured layer, the apertures going through the whole thickness of the layer, e.g. expanded metal, perforated layer, slit layer regular cells B32B3/12} - B32B 7/04: Interconnection of layers - B32B 7/08: by mechanical means - B32B 7/12: using interposed adhesives or interposed materials with bonding properties - B32B 15/043: {of metal (B32B15/01 takes precedence)} - B32B 15/20: comprising aluminium or copper {(B32B15/016 and B32B15/017 take precedence)} - B32B 27/08: of synthetic resin - B32B 27/32: comprising polyolefins {(comprising vinyl (co)polymers or acrylic (co)polymers B32B27/30)} - B32B 27/322: {comprising halogenated polyolefins, e.g. PTFE} - B32B 27/325: {comprising polycycloolefins} - B65B 69/005: {by expelling contents, e.g. by squeezing the container} - B01L 2300/044: pierceable, e.g. films, membranes - B01L 2400/0481: squeezing of channels or chambers - B01L 2400/0487: fluid pressure, pneumatics - B32B 2250/02: 2 layers - B32B 2250/03: 3 layers - B32B 2250/44: Number of layers variable across the laminate - B32B 2307/724: Permeability to gases, adsorption - B32B 2307/7246: Water vapor barrier - B32B 2307/7265: Non-permeable - B32B 2307/732: Dimensional properties - B32B 2439/80: Medical packaging - B32B 2535/00: Medical equipment, e.g. bandage, prostheses or catheter - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM - B65B: MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING (bundling and pressing devices for cigars A24C1/44; devices for tensioning and securing binders adapted to be supported by the article or articles to be bound B25B25/00; applying closure members to bottles, jars or similar containers B67B1/00-B67B6/00; concurrent cleaning, filling and closing of bottles B67C7/00; emptying bottles, jars, cans, casks, barrels or similar containers B67C9/00) ## Abstract The present disclosure relates to new types of fluid packaging devices and methods of making and using such fluid packaging devices. In various embodiments, the present disclosure provides devices, systems, and methods for packaging fluids. In various embodiments, the present disclosure provides a device including a storage compartment; a fluid stored in the storage compartment; a first aperture on the storage compartment; and a second aperture on the storage compartment. The first aperture is initially sealed or closed before the use of the device and configured to be opened to receive a pneumatic pressure. The second aperture is initially sealed or closed before the use of the device and configured to be opened to allow the fluid to exit or enter the storage compartment via the opened second aperture. ## Claims ### Claim 1 A fluid cartridge device comprising a packaging device, said packaging device comprising: a storage compartment; a fluid stored in the storage compartment; and an aperture on the storage compartment, the aperture being initially sealed or closed before the use of the packaging device and being configured to be opened to allow the fluid to exit or enter the storage compartment via the opened aperture; wherein: the packaging device is configured to transfer at least a portion of the fluid stored in the storage compartment to form a sample mixture with at least a portion of a sample received in the fluid cartridge device; and the packaging device is configured to transfer at least a portion of the sample mixture back into the storage compartment via the opened aperture, such that the packaging device functions both as a storage device and as a mixing device. ### Claim 2 (depends on 1) The fluid cartridge device of claim 1, wherein the aperture comprises a reversible seal. ### Claim 3 (depends on 1) The fluid cartridge device of claim 1, wherein the aperture is configured to be opened to allow the fluid to exit or enter the storage compartment via the opened aperture by an internal actuation component inside the storage compartment. ### Claim 4 (depends on 3) The fluid cartridge device of claim 3, wherein the internal actuation component is configured to open the aperture by compressing a part of the packaging device. ### Claim 5 (depends on 1) The fluid cartridge device of claim 1, wherein a gas-liquid interface or a vacuum-liquid interface is initially positioned above the aperture along a gravity direction. ### Claim 6 (depends on 1) The fluid cartridge device of claim 1, wherein: the packaging device comprises an additional aperture on the storage compartment; and the additional aperture is initially sealed or closed before the use of the packaging device. ### Claim 7 (depends on 6) The fluid cartridge device of claim 6, wherein: the additional aperture comprises an intact area of a barrier layer; and the additional aperture is configured to be pierced open. ### Claim 8 (depends on 6) The fluid cartridge device of claim 6, wherein the additional aperture is configured to be opened to receive a pneumatic pressure. ### Claim 9 (depends on 6) The fluid cartridge device of claim 6, wherein: the additional aperture is positioned higher than the aperture along a gravity direction; and a gas-liquid interface or a vacuum-liquid interface is initially positioned between the additional aperture and the aperture along the gravity direction. ### Claim 10 (depends on 6) The fluid cartridge device of claim 6, further comprising a microfluidic channel, wherein the microfluidic channel separates the additional aperture from the fluid stored in the storage compartment. ### Claim 11 (depends on 6) The fluid cartridge device of claim 6, further comprising a microfluidic channel, wherein the microfluidic channel is smaller than the additional aperture in a dimension measurement selected from the group consisting of width and height. ### Claim 12 A packaging device, comprising: a storage compartment; a fluid stored in the storage compartment; a first aperture on the storage compartment, the first aperture being initially sealed or closed before the use of the packaging device; a second aperture on the storage compartment, the second aperture being initially sealed or closed before the use of the packaging device and being configured to be opened to allow the fluid to exit or enter the storage compartment via the opened second aperture; and an internal actuation component in the storage compartment; wherein: the second aperture comprises a reversible seal; and the internal actuation component is configured to open the second aperture. ### Claim 13 (depends on 12) The packaging device of claim 12, wherein the internal actuation component is configured to break the reversible seal by compressing a part of the packaging device. ### Claim 14 (depends on 12) The packaging device of claim 12, wherein a gas-liquid interface or a vacuum-liquid interface is initially positioned above the second aperture along a gravity direction. ### Claim 15 (depends on 12) The packaging device of claim 12, wherein: the first aperture is positioned higher than the second aperture along a gravity direction; and a gas-liquid interface or a vacuum-liquid interface is initially positioned between the first aperture and the second aperture along the gravity direction. ### Claim 16 (depends on 12) The packaging device of claim 12, further comprising a microfluidic channel, wherein the microfluidic channel separates the first aperture from the fluid stored in the storage compartment. ### Claim 17 (depends on 12) The packaging device of claim 12, further comprising a microfluidic channel, wherein the microfluidic channel is smaller than the first aperture in a dimension measurement selected from the group consisting of width and height. ### Claim 18 (depends on 12) The packaging device of claim 12, wherein: the first aperture comprises an intact area of a barrier layer; and the first aperture is configured to be pierced open. ### Claim 19 (depends on 12) The packaging device of claim 12, wherein the first aperture is configured to be opened to receive a pneumatic pressure. ### Claim 20 (depends on 12) The packaging device of claim 12, wherein: the packaging device is configured to transfer at least a portion of the fluid stored in the storage compartment to form a sample mixture with at least a portion of a sample received in a fluid cartridge device; and the packaging device is configured to transfer at least a portion of the sample mixture back into the storage compartment via the opened aperture, such that the packaging device functions both as a storage device and as a mixing device.