# US 12,179,202 B2 · Microfluidic sorting devices and methods > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,179,202 B2 - Document ID: US12179202B2 - Status: Granted - Kind: B2 - Application number: 18/050,316 - Filed: 2022-10-27 - Published: 2023-10-12 - Granted: 2024-12-31 - Earliest claimed: 2016-09-20 - Assignees: European Molecular Biology Laboratory - Applicants: European Molecular Biology Laboratory - Inventors: Christoph A. Merten; Daniel Frenzel - Web page: https://patentgrep.com/patent/US12179202B2 ## CPC - B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules} - B03C 5/026: {using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]} - B01L 2200/0652: Sorting or classification of particles or molecules - B01L 2200/143: Quality control, feedback systems - B01L 2300/0861: Configuration of multiple channels and/or chambers in a single devices - B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting - B01L 2400/0424: Dielectrophoretic forces - B03C 2201/26: for use in medical or biological applications - G01N 15/149: specially adapted for sorting particles, e.g. by their size or optical properties - G01N 2015/0038: {Investigating nanoparticles} - G01N 2015/1006: {for cytology} - G01N 2015/1028: {Sorting particles} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention relates to the field of microfluidics and in particular to devices and methods for sorting objects in microfluidic channels. These devices and methods allow for fast and robust sorting in two-way and multi-way setups. They also enable sorting over extended periods of time. ## Claims ### Claim 1 A method for sorting an object, comprising the steps of (i) feeding an object into the microfluidic device comprising at least one sorting junction comprising (i) one stem channel, (ii) at least two branch channels, and (iii) an electrode pair, (ii) detecting a signal emitted from the object, (iii) switching on the electrode pair of the at least one sorting junction of the microfluidic device, (iv) passing the object across the at least one sorting junction, whereby the object enters one branch channel depending on the signal detected, wherein the electrode pair consists of a power electrode and a ground electrode, and wherein the power electrode of the electrode pair is located between the ground electrode and the stem channel and optionally a branch channel of the at least two branch channels. ### Claim 2 (depends on 1) The method of claim 1, wherein the electrode pair is located laterally of the stem channel and the branch channels. ### Claim 3 (depends on 1) The method of claim 1, wherein the electrode pair is located above or below the stem channel and the branch channels. ### Claim 4 (depends on 1) The method of claim 1, wherein at least a length section of both the power electrode and of the ground electrode of the electrode pair runs parallel to at least a part of the one stem channel up to and along at least a part of one of the at least two branch channels. ### Claim 5 (depends on 1) The method of claim 1, wherein the microfluidic device comprises at least two sorting junctions in a sequential order, wherein each sorting junction comprises two branch channels, and wherein the branch channel of one sorting junction is the stem channel of the subsequent sorting junction. ### Claim 6 (depends on 1) The method of claim 1, comprising one or more of a detector capable of detecting a signal upstream of the at least one sorting junction, wherein the detector is directly or indirectly connected to the electrode pair; and/or an aspiration means connected to a channel outlet of the microfluidic device, wherein the channel outlet receives fluid from a branch channel, and wherein the aspiration means generates flow pulses; and/or a multiwell plate, wherein at least one branch channel of the at least one sorting junction is connected to a well of the multiwell plate. ### Claim 7 (depends on 1) The method of claim 1, wherein one branch channel of the at least one sorting junction ultimately leads to a waste collector and wherein the electrode pair for this branch channel is switched on after the object has entered another branch channel of the sorting junction; and/or objects are sorted for at least 1 hour; and/or wherein flow resistance in the branch channels of the at least one sorting junction changes during sorting; and/or wherein the object is a microfluidic droplet containing a protein solution; and/or wherein fluid is aspirated from at least one branch channel in a pulsing rhythm; and/or wherein the object is sorted into a well of a multiwell plate. ### Claim 8 (depends on 7) The method of claim 7, wherein a collection electrode pair is switched on whenever a droplet is showing a desired readout signal, while the and a waste electrode is switched on whenever a droplet shows a non-desired readout signal, wherein switching on or off is after a specific time delay after detecting the desired readout signal. ### Claim 9 (depends on 8) The method of claim 8, wherein the time delay is calculated from the distance between the detection location and the sorting junction and the flow velocity at (or close to) the detection location. ### Claim 10 (depends on 1) The method of claim 1, wherein the microfluidic device comprises one of said electrode pair for each branch channel, wherein the stem channel is located between the electrode pairs, and wherein each of said electrode pairs is configured such that it directs an object into its branch channel. ### Claim 11 (depends on 1) The method of claim 1, wherein the at least one sorting junction comprises an electrode pair for each branch channel, and wherein in step (iii) one electrode pair of the at least one sorting junction of the microfluidic device is switched on. ### Claim 12 A method for sorting an object, comprising: (i) feeding an object into the microfluidic device comprising at least one sorting junction comprising (i) one stem channel, (ii) at least two branch channels, and (iii) an electrode pair, (ii) detecting a signal emitted from the object, (iii) switching on the electrode pair of the at least one sorting junction of the microfluidic device, (iv) passing the object across the at least one sorting junction, whereby the object enters one branch channel depending on the signal detected, wherein at least a length section of both electrodes of the electrode pair runs parallel to at least a part of the one stem channel up to and along at least a part of one of the at least two branch channels. ### Claim 13 (depends on 12) The method of claim 12, wherein the electrode pair is located laterally of the stem channel and the branch channels; or wherein the stem channel is located between electrodes of the electrode pair. ### Claim 14 (depends on 12) The method of claim 12, wherein the electrode pair is located above or below the stem channel and the at least two branch channels. ### Claim 15 (depends on 12) The method of claim 12, wherein the at least one sorting junction comprises an electrode pair for each branch channel, and wherein in step (iii) one electrode pair of the at least one sorting junction of the microfluidic device is switched on. ### Claim 16 (depends on 12) The method of claim 12, comprising one or more of a detector capable of detecting a signal upstream of the at least one sorting junction, wherein the detector is directly or indirectly connected to the electrode pair; and/or an aspiration means connected to a channel outlet of the microfluidic device, wherein the channel outlet receives fluid from a branch channel, and wherein the aspiration means generates flow pulses; and/or a multiwell plate, wherein at least one branch channel of the at least one sorting junction is connected to a well of the multiwell plate. ### Claim 17 (depends on 12) The method of claim 12, wherein one branch channel of the at least one sorting junction ultimately leads to a waste collector and wherein the electrode pair for this branch channel is switched on after the object has entered another branch channel of the sorting junction; and/or objects are sorted for at least 1 hour; and/or wherein flow resistance in the branch channels of the at least one sorting junction changes during sorting; and/or wherein the object is a microfluidic droplet containing a protein solution; and/or wherein fluid is aspirated from at least one branch channel in a pulsing rhythm; and/or wherein the object is sorted into a well of a multiwell plate. ### Claim 18 (depends on 17) The method of claim 17, wherein a collection electrode pair is switched on whenever a droplet is showing a desired readout signal, and a waste electrode is switched on whenever a droplet shows a non-desired readout signal, wherein switching on or off is after a specific time delay after detecting the desired readout signal. ### Claim 19 (depends on 18) The method of claim 18, wherein the time delay is calculated from the distance between the detection location and the sorting junction and the flow velocity at (or close to) the detection location. ### Claim 20 (depends on 12) The method of claim 12, wherein the microfluidic device comprises one of said electrode pair for each branch channel, wherein the stem channel is located between the electrode pairs, and wherein each of said electrode pairs is configured such that it directs an object into its branch channel.