# US 12,179,195 B2 · Bioprocessing fluid sensor arrangement > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,179,195 B2 - Document ID: US12179195B2 - Status: Granted - Kind: B2 - Application number: 17/281,727 - Filed: 2019-10-16 - Published: 2021-12-16 - Granted: 2024-12-31 - Earliest claimed: 2018-10-17 - Assignees: Cytiva Sweden AB - Applicants: Cytiva Sweden AB - Inventors: Klaus Gebauer - Web page: https://patentgrep.com/patent/US12179195B2 ## CPC - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 2200/141: Preventing contamination, tampering - B01L 2300/0663: Whole sensors - B01L 2300/0681: Filter - B01L 2400/0644: rotary valves - B01L 2400/0655: pinch valves - C12M 41/00: {Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N)} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract The present invention relates to a bioprocessing fluid sensor arrangement (100) for sensing fluidic properties in a process fluid path with a sensor (S), configured for aseptically connecting the sensor with at least one conditioning fluid while separating said sensor from the process fluid to at least one conditioning fluid e.g. for calibration, cleaning, regenerating and/or storing the sensor, the arrangement comprising a process fluid path (PF) having a process fluid inlet (PI) and a process fluid outlet (PO); a sensor (S) arranged in the process fluid path (PF); a bypass fluid path (BF) in the process fluid path (PF), for bypassing the sensor (S); a conditioning or cleaning fluid path (CF) having an inlet (CI) and an outlet (CO) each aseptically and fluidically connected to the process fluid path (PF), one on each side of the sensor (S); and flow controls (FC) for controlling the flow of fluids, whereby fluids can be controlled to flow either in the process fluid path (PF) via the sensor (S), or in the bypass fluid path (BF) omitting the sensor from the fluid path (PF), or in the conditioning or cleaning fluid path (CF) including the sensor in said flow but omitting the remaining process fluid path (PF) and bypass fluid path (BF). ## Claims ### Claim 1 A bioprocessing fluid sensor arrangement for sensing fluidic properties in a process fluid path with a sensor, configured for aseptically connecting the sensor with at least one conditioning fluid while separating said sensor from the process fluid to at least one conditioning fluid e.g. for calibration, cleaning, regenerating and/or storing the sensor, the arrangement comprising: a process fluid path having a process fluid inlet and a process fluid outlet; a sensor arranged in the process fluid path; a bypass fluid path in the process fluid path, for bypassing the sensor, comprising a bypass inlet and a bypass outlet, one on each side of the sensor; a conditioning or cleaning fluid path having an inlet and an outlet each aseptically and fluidically connected to the process fluid path, one on each side of the sensor, and each positioned between the bypass inlet and the bypass outlet; and flow controls comprising a processor programmed for controlling the flow of fluids, whereby fluids can be controlled to flow in each of a) the process fluid path via the sensor, b) the bypass fluid path omitting the sensor from the fluid path, and c) the conditioning or cleaning fluid path including the sensor in said flow but omitting other portions of the process fluid path and bypass fluid path. ### Claim 2 (depends on 1) The sensor arrangement according to claim 1, wherein: the bypass fluid path comprises flow controls in the form of a bypass inlet valve coupled to the process fluid inlet and an inlet of the sensor, the process fluid path comprises flow controls in the form of a process fluid inlet valve coupled between the process fluid inlet and the inlet of the sensor and a process fluid outlet valve coupled between an outlet of the sensor and the process fluid outlet, the conditioning or cleaning fluid path comprises flow controls in the form of a conditioning fluid inlet valve and a conditioning fluid outlet valve, wherein the conditioning fluid inlet valve, the conditioning fluid outlet valve and the bypass inlet valve are configured to stop fluid to flow through the valves or to allow fluid to flow through the valves, and wherein the process fluid inlet valve and the process fluid outlet valve are configured to allow fluid to flow through the valves or configured to stop fluid to flow through the valves. ### Claim 3 (depends on 1) The sensor arrangement according to claim 1, wherein the conditioning or cleaning fluid path further comprises a first sterile filter. ### Claim 4 (depends on 1) The sensor arrangement according to claim 1, further comprising a second sensor, the second sensor: being arranged in the process fluid path, or being arranged in the bypass fluid path. ### Claim 5 (depends on 1) The sensor arrangement according to claim 1, wherein the bypass fluid path further comprises a first ventilator. ### Claim 6 (depends on 1) The sensor arrangement according to claim 1, wherein a bypass fluid path of the internal coupling network further comprises a first sterile filter. ### Claim 7 (depends on 1) The sensor arrangement according to claim 1, further comprising a second sensor of the same type as the sensor, the second sensor: being arranged in a process fluid path, or being arranged in a bypass fluid path. ### Claim 8 (depends on 1) The sensor arrangement according to claim 1, wherein a bypass fluid path of the internal coupling network further comprises a first ventilator. ### Claim 9 (depends on 1) A bioprocessing sensor system configured for aseptic sensor conditioning, the system comprising: a processor, and a sensor arrangement according to claim 1, wherein flow controls and sensors of a coupling network of the sensor arrangement are communicatively coupled to the control unit and configured to operate in an operational mode or to operate in a conditioning mode in response to control signals received from the processor. ### Claim 10 (depends on 1) A method performed by a bioprocessing sensor system configured for aseptic sensor conditioning, the method comprising: receiving input indicative of a desire to condition the sensor arrangement according to claim 1, sending control signals from a processor to one or more flow controls indicating to the flow controls to operate in a conditioning mode, conditioning a sensor, sending control signals from the control unit to the one or more flow controls of the sensor arrangement or the sensor unit indicating to the valves to operate in an operational mode. ### Claim 11 (depends on 10) The method of claim 10, further comprising receiving measurements from a second sensor when operating in the conditioning mode. ### Claim 12 A bioprocessing sensor unit for sensing fluidic properties in a process fluid path and further configured for aseptic sensor connection to additional conditioning or cleaning or bypass fluid paths, comprising: a body, process couplers, coupled to the body and configured to provide aseptic connection via a process fluid inlet and a process fluid outlet of the sensor unit, a bypass inlet and a bypass outlet positioned one on each side of the sensor unit, conditioning couplers coupled to the body and configured to provide aseptic connection via a conditioning fluid inlet and a conditioning fluid outlet of the sensor unit, the conditioning fluid inlet and the conditioning fluid outlet positions one on each side of the sensor unit and each positions between the bypass inlet and the bypass outlet, an internal coupling network comprising fluid paths and one or more flow controls comprising a processor programmed for controlling the flow of fluids in the fluid paths, the internal coupling network configured to operate in each of: a) an operational mode, where the flow controls of the internal coupling network are configured to allow fluid to flow from the process fluid inlet to the process fluid outlet via a sensor, and b) a conditioning mode, where the flow controls of the internal coupling network are configured to allow fluid to flow from the conditioning fluid inlet to the conditioning fluid outlet via the sensor or to flow from a calibration fluid inlet to a calibration fluid outlet via the sensor. ### Claim 13 (depends on 12) The sensor unit according to claim 12, wherein: a bypass fluid path of the internal coupling network comprises a bypass inlet valve coupled to the process fluid inlet and an inlet of the sensor, a process fluid path of the internal coupling network comprises a process fluid inlet valve coupled between the process fluid inlet and the inlet of the sensor and a process fluid outlet valve coupled between an outlet of the sensor and the process fluid outlet, a conditioning or cleaning fluid path of the internal coupling network comprises a conditioning fluid inlet valve and a conditioning fluid outlet valve, wherein the conditioning fluid inlet valve, the conditioning fluid outlet valve and the bypass inlet valve are configured to stop fluid to flow through the valves in the operational mode and configured to allow fluid to flow through the valves in the conditioning mode, and wherein the process fluid inlet valve and the process fluid outlet valve are configured to allow fluid to flow through the valves in the operational mode and configured to stop fluid to flow through the valves in the conditioning mode. ### Claim 14 (depends on 1) A processor programmed to execute instructions stored on a non-transitory computer-readable memory for causing a sensor arrangement; where the instructions are executed by the processor comprised in the sensor arrangement, to perform the method steps of claim 1. ### Claim 15 (depends on 14) A non-transitory computer-readable medium having stored thereon computer executable instructions having the program according to claim 14 embodied therein.