# US 12,179,155 B2 · Device for bind and elute chromatography using membranes, and method of manufacture

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- Number: US 12,179,155 B2
- Document ID: US12179155B2
- Status: Granted
- Kind: B2
- Application number: 17/432,122
- Filed: 2020-02-20
- Published: 2022-02-03
- Granted: 2024-12-31
- Earliest claimed: 2019-02-20
- Assignees: EMD Millipore Corporation
- Applicants: Merck Millipore Ltd.
- Inventors: Kevin Rautio; Sean Foley; Stephen G. Hunt; Bumchul Lee; Nathan Landry
- Web page: https://patentgrep.com/patent/US12179155B2

## CPC

- B01D 63/084: {at least one flow duct intersecting the membranes}
- B01D 63/081: {Manufacturing thereof}
- B01D 2313/042: Adhesives or glues
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)

## Abstract

Single-use integral chromatography unit having an inlet and an outlet, and comprising one or more plates or pairs of filter plates interposable between a pair of end plates. In certain embodiments, each of the filter plates comprises a polymeric framework with one or more membranes supported therein. The filter plates and end plates may be assembled to form a substantially fixed, substantially water-tight, integral stack. Fluid entering the unit through a common inlet passes the membrane or membranes of each filter plate substantially contemporaneously prior to exiting the unit through a common outlet (cf, “parallel” flow). The assembly is a modular design, as multiple pairs of plates can be stacked in a suitable holder to form a single chromatography unit.

## Claims

### Claim 1

A filter plate having an inlet and an outlet, said filter plate disposed between two end plates, comprising a polymeric framework wherein said polymeric framework comprises a polyphenylene ether/polystyrene blend having a flat filtration zone wherein said flat filtration zone further comprises a low-shrinkage seal comprising a polystyrene material or a polyphenylene ether/polystyrene blend material, sealing a plurality of stacked membranes to said polymeric framework in said flat filtration zone, having a radio-frequency weld between the two end plates and the filter plate.

### Claim 2 (depends on 1)

The filter plate of claim 1, further comprising a plurality of apertures in fluid communication with said outlet.

### Claim 3 (depends on 2)

The filter plate of claim 2, wherein said apertures are positioned such that during a filtration operation, filtrate through said plurality of stacked membrane enters said apertures and flows to said outlet.

### Claim 4 (depends on 1)

The filter plate of claim 1, wherein said plurality of stacked membranes is overmolded to said polymeric framework.

### Claim 5

A bind and elute chromatography unit having an inlet and an outlet, and comprising at least one pair of filter plates interposed between a pair of end plates; each of said filter plates of said at least one pair of filter plates comprising a polymeric framework having a filtration zone and one or more membranes bonded to said polymeric framework in said filtration zone wherein said polymeric framework comprises a polyphenylene ether/polystyrene blend wherein said flat filtration zone further comprises a low-shrinkage seal comprising a polystyrene material or a polyphenylene ether/polystyrene blend material, sealing a plurality of stacked membranes; the filter plates of said at least one pair of filter plates being placed in back-to-back relation thereby creating a channel between the membrane or membranes in a first plate of said pair and the membrane or membranes in a second plate of said pair, into which fluid flows from said inlet and exits through said outlet after passing through the membrane or membranes in each of said first and second plates, further comprising a plurality of spaced ribs provided within said flat filtration zone to support the plurality of stacked membranes and provide flow passageways to channel fluid towards a plurality of apertures.

### Claim 6 (depends on 5)

The chromatography unit of claim 5, wherein there are a plurality of stacked membranes in each of said flat filtration zones of each plate of said at least one pair of plates.

### Claim 7 (depends on 5)

The chromatography unit of claim 5, wherein said pair of plates are RF welded together.