# US 12,178,414 B1 · Gingival crevicular fluid absorbing brush

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- Number: US 12,178,414 B1
- Document ID: US12178414B1
- Status: Granted
- Kind: B1
- Application number: 18/661,927
- Filed: 2024-05-13
- Granted: 2024-12-31
- Earliest claimed: 2023-08-03
- Assignees: King Faisal University
- Applicants: King Faisal University
- Inventors: Zohaib Khurshid; Syed Akhtar Bokhari; Muhammad Sohail Zafar; Syed Faraz Moin; Shariq Najeeb
- Web page: https://patentgrep.com/patent/US12178414B1

## CPC

- A61B 10/0045: {Devices for taking samples of body liquids}
- A61B 10/0051: {for taking saliva or sputum samples (devices for receiving spittle A61J9/00)}
- A61B 2010/0216: {Sampling brushes}
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)

## Abstract

An instrument and method for collecting and harvesting gingival crevicular fluid samples in a noninvasive manner is provided. Once optimally positioned, an absorbing brush of the instrument is inserted into the gingival sulcus of a patient and held in place while the bristles of the absorbing brush absorb the gingival crevicular fluid. Once removed from the collection sight, the absorbing brush bristles can provide a visual indication of the presence of biomarkers and proteins associated with periodontitis.

## Claims

### Claim 1

A method for collecting or harvesting a Gingival Crevicular Fluid (GCF) sample from a gingival sulcus of a patient, comprising:
inserting a new absorbing brush holder into a head of a hand-held instrument;
grasping an inclined handle which terminates in a main horizontal segment of said hand-held instrument;
positioning an inclined segment of said hand-held instrument within an oral cavity of said patient, said inclined segment being adjacent to said main horizontal segment at an end opposite said inclined handle;
positioning said head of said hand-held instrument such that an absorbing brush is inserted into said gingival sulcus of said patient;
holding said head of said hand-held instrument in place such that said absorbing brush absorbs said GCF sample resident within said gingival sulcus;
retracting said head of said hand-held instrument such that said absorbing brush exits said gingival sulcus of said patient;
removing said inclined segment from said oral cavity of said patient;
detecting a visual indication in said absorbing brush of a presence of biomarkers or proteins associated with periodontitis; and
removing said absorbing brush holder from said head of said hand-held instrument and placing said absorbing brush holder in a container.

### Claim 2 (depends on 1)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 1, wherein said head has an opening commensurate in size with a size of said absorbing brush holder such that said absorbing brush holder is insertable into said opening of said head.

### Claim 3 (depends on 2)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 2, wherein said absorbing brush has a conical shape and is made of plurality of bristles.

### Claim 4 (depends on 3)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 3, wherein said plurality of bristles are made of specially treated cotton fibers.

### Claim 5 (depends on 4)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 4, wherein said visual indication is when said plurality of bristles change to a visible red color when said presence of said biomarkers and said proteins associated with said periodontitis are detected.

### Claim 6 (depends on 5)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 5, wherein said container further includes a buffer to stabilize said biomarkers and said proteins.

### Claim 7 (depends on 6)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 6, wherein said plurality of bristles are made of said specially treated cotton fibers.

### Claim 8 (depends on 7)

The method for collecting or harvesting said GCF sample from said gingival sulcus of said patient as recited in claim 7, wherein said plurality of bristles change to said visible red color when said presence of said biomarkers and said proteins associated with said periodontitis are detected.