# US 12,178,204 B2 · Low-temperature damage-relieving agent or necrosis inhibitor, and method for preserving living organism, tissue or cell

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- Number: US 12,178,204 B2
- Document ID: US12178204B2
- Status: Granted
- Kind: B2
- Application number: 16/636,592
- Filed: 2018-08-01
- Published: 2020-11-26
- Granted: 2024-12-31
- Earliest claimed: 2017-08-08
- Assignees: Shin Nippon Yakugyo Co., Ltd.
- Applicants: Shin Nippon Yakugyo Co., Ltd.
- Inventors: Hidehisa Kawahara; Eri Tagawa
- Web page: https://patentgrep.com/patent/US12178204B2

## CPC

- A01N 1/125: Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
- A01N 1/126: Physiologically active agents, e.g. antioxidants or nutrients
- A01N 1/162: Temperature processes, e.g. following predefined temperature changes over time
- A23F 5/26: Extraction of water soluble constituents {(A23F5/246 takes precedence)}
- C12N 2511/00: Cells for large scale production
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23F: COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

An organ, a tissue or cells of a living body can be preserved stably at a non-freezing low temperature without damaging the organ, the tissue or the cells, by the method of preserving an organ, a tissue or cells of a living body by using the low temperature damage alleviating agent or the necrosis inhibitor comprising a coffee extract of the present invention, or a medium comprising a coffee extract.

## Claims

### Claim 1

A method of preserving an isolated organ, an isolated tissue or isolated cells, comprising preserving the isolated organ, the isolated tissue, or the isolated cells in a medium comprising coffee melanoidin at a concentration of about 0.001 to 0.5 mg/ml at a temperature of about −10 to 0° C. for up to 6 days.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the isolated organ, the isolated tissue or the isolated cells is preserved at a low temperature of about −8 to −4° C.

### Claim 3 (depends on 1)

The method according to claim 1, wherein ATP depletion of the isolated organ, the isolated tissue or the isolated cells during preservation is delayed.

### Claim 4 (depends on 1)

The method of claim 1, wherein the coffee melanoidin is a melanoidin having a molecular weight of 10,000 or more.

### Claim 5 (depends on 1)

The method of claim 1, wherein the coffee melanoidin is the melanoidin obtained by mixing an aqueous coffee extract with an organic solvent to perform an organic solvent extraction step that provides an organic fraction and obtaining the coffee melanoidin from the organic fraction.

### Claim 6 (depends on 1)

The method according to claim 1, comprising a step of preserving the isolated organ, the isolated tissue or the isolated cells in a medium comprising coffee melanoidin at a temperature of about −10 to 0° C. for 0 to 4 days.

### Claim 7 (depends on 1)

The method according to claim 1, wherein the coffee melanoidin is a compound chemically synthesized melanoidin.