# US 12,178,193 B2 · Method for mass rearing of Telenomus remus

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- Number: US 12,178,193 B2
- Document ID: US12178193B2
- Status: Granted
- Kind: B2
- Application number: 18/663,130
- Filed: 2024-05-14
- Published: 2024-11-28
- Granted: 2024-12-31
- Earliest claimed: 2023-05-26
- Assignees: Yunnan Tobacco Co., Ltd. Kunming Branch; Yunnan Pusher Biotechnology Co., Ltd.
- Applicants: Yunnan Tobacco Co., Ltd. Kunming Branch; Yunnan Pusher Biotechnology Co., Ltd.
- Inventors: Yonghui Xie; Zhengling Liu; Dekai Ning; Hongming Li; Zhonglong Lin; Jingyu Shi; Zhijiang Wang; Aimin Shi; Yu Shen; Youguo Zhan; Xiaofei Gu; Dingguo Shi; Facong Qian; Shiyou Deng; Duo Wang; Dongya Xu; Linqing Tian; Yuanshen Wang; Qinglin Rao; Xianlong Ding; Zhuoheng Li
- Web page: https://patentgrep.com/patent/US12178193B2

## CPC

- A01K 51/00: Appliances for treating beehives or parts thereof, e.g. for cleaning or disinfecting
- Y02P 60/87: Re-use of by-products of food processing for fodder production
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

A method for mass rearing of Telenomus remus includes the following steps: (1) establishing a Telenomus remus population of a Corcyra cephalonica strain; (2) collecting Corcyra cephalonica eggs laid by Corcyra cephalonica on the day, and fixing the Corcyra cephalonica eggs on a sticker; (3) inoculating female Telenomus remus of the Corcyra cephalonica strain in the step (1) into the Corcyra cephalonica eggs in the step (2); and (4) after 24 h to 48 h of a parasitism, taking parasitic Corcyra cephalonica eggs out, and feeding the parasitic Corcyra cephalonica eggs under the following conditions: a temperature: 26±1° C., a relative humidity: 65±5%, and a photoperiod: L:D=14:10, where long axes of the Corcyra cephalonica eggs in the step (2) have a length of 0.48 mm to 0.60 mm. The method of the present disclosure has great significance for the long-term control of pests of the Noctuidae family.

## Claims

### Claim 1

A method for a mass rearing of a Telenomus remus, comprising the following steps:
(1) establishing a Telenomus remus population of a Corcyra cephalonica strain,
wherein the step (1) comprises:
(a) preparing a crude extract of Spodoptera litura eggs;
(b) collecting first Corcyra cephalonica eggs laid by a Corcyra cephalonica on a day, and fixing the first Corcyra cephalonica eggs on a first sticker;
(c) adding the crude extract of the Spodoptera litura eggs prepared in the step (a) dropwise to the first Corcyra cephalonica eggs in the step (b), and inoculating a female Telenomus remus; and
(d) after 24 h to 48 h of a first parasitism, taking first parasitic Corcyra cephalonica eggs out, and feeding the first parasitic Corcyra cephalonica eggs in an artificial climate chamber;
(2) collecting second Corcyra cephalonica eggs laid by the Corcyra cephalonica on the day, and fixing the second Corcyra cephalonica eggs on a second sticker;
(3) inoculating a female Telenomus remus of the Corcyra cephalonica strain in the step (1) into the second Corcyra cephalonica eggs in the step (2); and
(4) after 24 h to 48 h of a second parasitism, taking second parasitic Corcyra cephalonica eggs out, and feeding the second parasitic Corcyra cephalonica eggs under the following conditions: a temperature: 26±1° C., a relative humidity: 65±5%, and a photoperiod: L:D=14:10,
wherein long axes of the second Corcyra cephalonica eggs in the step (2) have a length of 0.48 mm to 0.60 mm; and
operations of preparing the crude extract of the Spodoptera litura eggs in the step (a) are as follows: collecting eggs laid by a Spodoptera litura on the day, placing the eggs in a container, and adding a solvent to the container; sealing the container, gently shaking the container, and allowing the container to stand; and collecting a resulting supernatant from the container.

### Claim 2 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein a feed formula for feeding the Corcyra cephalonica in the step (2) comprises the following components: a flour, a wheat bran, a soybean powder, a yeast powder, and a multivitamin; and
the multivitamin comprises vitamin C, riboflavin, niacin, calcium pantothenate, pyridoxine, biotin, folic acid, cobalamin, and glucose.

### Claim 3 (depends on 2)

The method for the mass rearing of the Telenomus remus according to claim 2, wherein the feed formula for feeding the Corcyra cephalonica in the step (2) comprises the following components in weight percentages:
60% to 90% of the flour;
4% to 15% of the wheat bran;
4% to 20% of the soybean powder;
1% to 5% of the yeast powder; and
0.5% to 2% of the multivitamin.

### Claim 4 (depends on 3)

The method for the mass rearing of the Telenomus remus according to claim 3, wherein the vitamin C, the riboflavin, the niacin, the calcium pantothenate, the pyridoxine, the biotin, the folic acid, the cobalamin, and the glucose in the multivitamin are in a weight ratio of (8-20):(15-40):(35-60):(35-60):(8-20):(0.1-5):(8-20):(0.1-5):(700-800).

### Claim 5 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein the Spodoptera litura is a Spodoptera litura laying 500±50 eggs on the day, and a ratio of the female Telenomus remus to the first Corcyra cephalonica eggs is 1:(8-12).

### Claim 6 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein the solvent is n-hexane, and the solvent is added to the container in a volume of 2 μL to 10 μL.

### Claim 7 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein the female Telenomus remus of the Corcyra cephalonica strain inoculated in the step (3) is a female Telenomus remus of the Corcyra cephalonica strain undergoing emergence within 24 h.

### Claim 8 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein in the step (3), a ratio of the female Telenomus remus of the Corcyra cephalonica strain to the second Corcyra cephalonica eggs is 1:(8-12).

### Claim 9 (depends on 1)

The method for the mass rearing of the Telenomus remus according to claim 1, wherein a feed formula for feeding the Corcyra cephalonica in the step (2) comprises the following components in weight percentages:
60% to 90% of a flour;
4% to 15% of a wheat bran;
4% to 20% of a soybean powder;
1% to 5% of a yeast powder; and
0.5% to 2% of a multivitamin.