Microscopic imaging system and microscopic imaging method for sample angle recognition

A microscopic imaging method for sample angle recognition, applied to a microscopic imaging system that comprises:

• an object stage for carrying a sample, wherein an object to be analyzed in the sample having a polarized light response;
• a light source for providing an excitation light to excite the sample;
• a polarization rotation device for modulating a polarization angle of the excitation light to obtain a polarization-modulated excitation light, a polarization angle of the polarization-modulated excitation light being changed continuously and cyclically within a preset angle range;
• a beam splitting device for splitting the polarization-modulated excitation light into two parts including a first excitation light and a second excitation light;
• an imaging device for detecting an intensity of fluorescence or reflected light emitted by the object to be analyzed in the sample after being excited by the first excitation light, the intensity of fluorescence or reflected light being related to a polarization angle of the first excitation light;
• and
• a polarization detection device for detecting change information about a polarization angle of the second excitation light to obtain polarization change information of the polarization-modulated excitation light, the polarization detection device comprising a second polarizer through which the second excitation light passes;
• the method comprising:
• modulating a polarization angle of the excitation light emitted by the light source and outputting a polarization-modulated excitation light, a polarization angle of the polarization-modulated excitation light being changed continuously and cyclically within a preset angle range;
• obtaining from the imaging device the detected intensity of fluorescence or reflected light emitted by the object to be analyzed in the sample after being excited by the first excitation light to obtain a sample characteristic curve;
• in a case of the second polarizer being at different polarization angles, obtaining from the polarization detection device a detected change information about the polarization angle of the second excitation light so as to obtain a plurality of model characteristic curves, the model characteristic curves being corresponding to the polarization angles of the second polarizer one by one, and each of the model characteristic curves corresponding to the change information about the polarization angle of the second excitation light at one polarization angle;
• and
• calculating an error between each of the model characteristic curves and the sample characteristic curve, taking a polarization angle corresponding to a model characteristic curve with a minimum error compared with the sample characteristic curve as angle information about the object to be analyzed in the sample, and outputting the angle information about the object to be analyzed in the sample.