Compositions and analysis of dephosphorylated oligoribonucleotides

A composition comprising:

• (a) a human RNase 4 in an amount having catalytic activity equivalent to 100 to 10,000 units of RNase T1, wherein one unit of RNase T1 causes an increase in absorbance of 1.0 at 260 nm in 15 minutes when yeast RNA is hydrolyzed at 37° C. and pH 7.5 in a reaction comprising 50 mM Tris-HCl, 2 mM EDTA, and 3 mg/mL of the yeast RNA;
• (b) a bacterial RNA end repair enzyme or a bacteriophage RNA end repair enzyme;
• and
• (c) an RNA comprising at least 1,000 nucleotides in an amount of 0.01 μg to 100 μg, wherein the human RNase 4 specificity is selected from (1) cleavage after a specific nucleotide followed by a purine, (2) cleavage after a specific nucleotide followed by a pyrimidine,
• (3) cleavage after a purine followed by a specific nucleotide, and (4) cleavage after a pyrimidine followed by a specific nucleotide;
• and
• wherein the RNA end repair enzyme comprises phosphodiesterase and phosphomonoesterase activities.