Trehalose phosphorylase
AssigneesC-LEcta GmbH, New Matterhorn, LLC
InventorsBirgit Brucher, Andreas Vogel
Life of this patent
Estimating the term…
Abstract
The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.
Claims9
A trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 87% identical to
- (i) the amino acid sequence of SEQ ID NO:
- 81, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions are selected from the group consisting of amino acid positions L108, V112, N221, A300, T319, F345, P379, I483, V544, S550, Q558, A643, and L707 of SEQ ID NO:
- 81, or
- (ii) the amino acid sequence of SEQ ID NO:
- 160, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions are selected from the group consisting of amino acid positions L108, V112, N221, A300, T319, F345, P379, V483, V544, S550, Q558, A643, and L707 of SEQ ID NO:
- 160;
- and wherein
- (a) if the one or more amino acid substitutions comprises a substitution at position A300 of SEQ ID NO:
- 81 or SEQ ID NO:
- 160, then the amino acid substitution at position 300 is selected from the group consisting of A300G, A300I, A300M, A300P and A300V;
- (b) if the one or more amino acid substitutions comprises a substitution at amino acid position I483 of SEQ ID NO:
- 81, then the amino acid substitution at position 483 is selected from the group consisting of I483A, I483G, I483L, I483M, and I483P;
- and
- (c) if the one or more amino acid substitutions comprises a substitution at amino acid position V483 of SEQ ID NO:
- 160, then the amino acid substitution at position 483 is selected from the group consisting of V483A, V483G, V483L, V483M, and V483P.
A trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 87% identical to an amino acid sequence of SEQ ID NO:
- 81 or SEQ ID NO:
- 160, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein
- (a) the one or more amino acid positions is selected from the group consisting of amino acid positions 108, 112, 221, 300, 319, 345, 379, 483, 544, 550, 558, 643, and 707 of SEQ ID NO:
- 81 or SEQ ID NO:
- 160;
- (b) if the one or more amino acid substitutions comprises a substitution at amino acid position I483 of SEQ ID NO:
- 81, then the amino acid substitution at position 483 is selected from the group consisting of I483A, I483G, I483L, I483M, and I483P;
- and
- (c) if the one or more amino acid substitutions comprises a substitution at amino acid position V483 of SEQ ID NO:
- 160, then the amino acid substitution at position 483 is selected from the group consisting of V483A, V483G, V483L, V483M, and V483P,
- wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid sequence according to any one of SEQ ID NO:
- 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, and 189.
Dependencies are inferred from the claim text.