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US 12,173,329 B2Granted
Acyl-ACP reductase with improved properties
AssigneeGenomatica, Inc.
InventorsMathew Rude, Na Trinh, Andreas Schirmer, Jacob Gano
Life of this patent
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Abstract
The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Claims20
A variant acyl-ACP reductase (AAR) polypeptide comprising at least 85% sequence identity to the amino acid sequence of SEQ ID NO:
- 81, wherein said variant AAR polypeptide comprises one or more mutations at an amino acid position corresponding to position 8, 10, 11, 16, 17, 18, 19, 21, 22, 23, 24, 31, 34, 38, 43, 50, 58, 63, 64, 65, 83, 86, 96, 112, 113, 116, 117, 118, 120, 135, 148, 150, 153, 154, 155, 157, 159, 168, 172, 177, 179, 180, 187, 188, 191, 209, 210, 211, 236, 238, 253, 274, 277, 281, 282, 283, 284, 285, 286, 291, 316, 324, 328, 335, and 337 of SEQ ID NO:
- 81, and wherein said variant AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.
Dependencies are inferred from the claim text.