Heterologous expression of phosphite dehydrogenase in picochlorum spp
AssigneeAlliance for Sustainable Energy, LLC
InventorsLukas Royce Dahlin, Michael T. Guarnieri
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Abstract
Phosphite dehydrogenase (ptxD) expression was established as a selectable marker for nuclear and chloroplast genetic selection in Picochlorum renovo and Picochlorum celeri Phosphite was used as a sole phosphorus source in P. renovo and P. celeri. Growth on phosphite led to comparable growth and composition relative to phosphate.
Claims18
A non-naturally occurring Picochlorum spp. capable of growth on phosphite as a sole phosphorus source wherein the Picochlorum spp. comprises a mutated ptxD gene with greater than 95% sequence identity to SEQ ID NO:
- 1.
A non-naturally occurring Picochlorum spp. capable of growth on phosphite as a sole phosphorus source comprising a mutated ptxD gene with greater than 95% sequence identity to SEQ ID NO:
- 1 and wherein the expressed enzyme from the mutated ptxD gene is capable of NADP+ and NAD+ utilization and wherein the non-naturally occurring Picochlorum spp. further comprises an exogenous phosphite-specific transporter.
A non-naturally occurring Picochlorum spp. capable of growth on phosphite as a sole phosphorus source comprising a mutated ptxD gene with greater than 90% sequence identity to SEQ ID NO:
- 3 and wherein the expressed enzyme from the mutated ptxD gene is capable of NADP+ and NAD+ utilization and wherein the non-naturally occurring Picochlorum spp. further comprises an exogenous phosphite-specific transporter.
Dependencies are inferred from the claim text.