Recombinant rotavirus expression system and recombinant rotaviruses
AssigneeThe Trustees of Indiana University
InventorsJohn Thomas Patton, Asha Ann Philip
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Abstract
Embodiments herein report compositions, methods, uses and manufacturing procedures for rotavirus constructs and immunogenic compositions thereof. Some embodiments concern compositions that include, but are not limited to, chimeric rotaviruses of use in immunogenic compositions against rotavirus infection as well as against other pathogenic virus infection in a subject. In certain embodiments, constructs of use herein can be generated and used where a rotavirus expression system further includes one or more nucleic acid molecules encoding one or more polypeptides of another pathogen (e.g. another enteric or mucosal pathogen).
Claims17
A recombinant rotavirus comprising a gene segment including a nucleotide sequence encoding an intact, full-length rotavirus nonstructural protein 3 (NSP3) and a nucleotide sequence encoding a non-rotavirus polypeptide, wherein the NSP3 and the non-rotavirus polypeptide are encoded by a single open reading frame, separated by a self-cleavage protease domain.
A recombinant rotavirus expression system comprising:
- a nonstructural protein 3 (NSP3) expression vector including a nucleotide sequence encoding an intact, full-length rotavirus (NSP3) and a nucleotide sequence encoding a non-rotavirus polypeptide, wherein the NSP3 and the non-rotavirus polypeptide are encoded by a single open reading frame, separated by a self-cleavage protease domain;
- a VP1 expression vector;
- VP2 expression vector;
- a VP3 expression vector;
- a VP4 expression vector;
- a VP6 expression vector;
- a VP7 expression vector;
- a NSP1 expression vector;
- a NSP2 expression vector;
- a NSP4 expression vector;
- a NSP5/6 expression vector;
- and
- an African Swine Fever Virus NP868R RNA capping enzyme expression vector.
Dependencies are inferred from the claim text.