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US 12,172,163 B2Granted
Hydrodynamic focusing apparatus and methods
AssigneeCytonome/St, LLC
InventorsErin Koksal, Johnathan Charles Sharpe, Kristopher Scott Buchanan, Blair D. Morad
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Abstract
A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.
Claims20
A microfluidic assembly for use with a particle processing instrument, the microfluidic assembly comprising:
- a substrate;
- and
- a flow channel formed in the substrate, the flow channel having:
- an inlet configured to receive a single sample stream;
- a lateral fluid focusing feature to focus the single sample stream laterally;
- a first and a second vertical fluid focusing feature to focus the single sample stream vertically, the lateral, the first, and the second vertical fluid focusing features provided at different longitudinal locations along the flow channel, the second vertical fluid focusing feature located downstream of the first vertical focusing feature;
- wherein a bottom surface of the flow channel lies in a first plane upstream of the first and second vertical fluid focusing features and the bottom surface of the flow channel shifts vertically upward to lie in a second plane downstream of the first and second vertical focusing features;
- and
- an inspection region at least partially downstream of the lateral fluid focusing feature, the first vertical focusing feature, and the second vertical focusing feature.
6 more elements
Dependencies are inferred from the claim text.