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US 12,172,156 B2Granted
Flow cells
AssigneeIllumina, Inc.
InventorsLewis J. Kraft, Tarun Kumar Khurana, Yir-Shyuan Wu, Xi-Jun Chen, Arnaud Rival, Justin Fullerton, M. Shane Bowen, Hui Han, Jeffrey S. Fisher, Yasaman Farshchi, Mathieu Lessard-Viger
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Abstract
An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.
Claims6
A method, comprising:
- introducing a plurality of complexes to a flow cell including:
- a plurality of chambers, each having a depth that is at least about 50% of an average diameter of the complexes, and wherein each chamber has a bottom surface;
- and
- primers attached to the bottom surface within each of the plurality of chambers;
- wherein each of the plurality of complexes includes:
- a carrier;
- and
- sequencing-ready nucleic acid fragments from the same template nucleic acid attached to or contained within the carrier;
- whereby at least some of the complexes become trapped in at least some of the plurality of chambers;
- washing away non-trapped complexes from the flow cell;
- and
- without introducing an external immobilizing agent to the flow cell, causing the carrier of the trapped complexes to release the sequencing-ready nucleic acid fragments into the respective chamber in which each complex is trapped, whereby transport and seeding of the sequencing-ready nucleic acid fragments are restricted by the depth.
9 more elements
Dependencies are inferred from the claim text.