Recombinant rhabdovirus encoding for a CD80 extracellular domain Fc-fusion protein
AssigneeBoehringer Ingelheim International GmbH
InventorsPhilipp Mueller, Klaus Erb, Patrik Erlmann, Guido Wollmann
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Abstract
The present invention relates to the field of oncolytic viruses and in particular to a recombinant rhabdovirus, such as vesicular stomatitis virus encoding in its genome for a CD80 extracellular domain Fc-fusion protein. The invention is further directed to the use of the recombinant virus in the treatment of cancer, and also to methods for producing such viruses.
Claims15
A recombinant vesicular stomatitis virus encoding in its genome at least one CD80 extracellular domain Fc-fusion protein, wherein the CD80 extracellular domain Fc-fusion protein comprises the extracellular domain of CD80 and further comprises the Fc domain of an IgG wherein the CD80 extracellular domain Fc-fusion protein comprises SEQ ID NO:
- 4;
- and
- the gene coding for the wild-type glycoprotein G of the recombinant vesicular stomatitis virus is replaced by the gene coding for the glycoprotein G (GP) of Lymphocyte choriomeningitis virus (LCMV), and/or the wild-type glycoprotein G is replaced by the glycoprotein G (GP) of LCMV.
A recombinant vesicular stomatitis virus encoding in its genome a vesicular stomatitis virus nucleoprotein (N), large protein (L), phosphoprotein (P), matrix protein (M), glycoprotein (G) and at least one CD80 extracellular domain Fc-fusion protein, wherein the CD80 extracellular domain Fc-fusion protein comprises
- a CD80 extracellular domain Fc-fusion protein, comprising SEQ ID NO:4, wherein, the gene coding for the wild-type glycoprotein G of the vesicular stomatitis virus is replaced by the gene coding for the glycoprotein G (GP) of lymphocyte choriomeningitis virus (LCMV), and/or the wild-type glycoprotein G is replaced by the glycoprotein G (GP) of LCMV, and wherein
- the nucleoprotein (N) comprising an amino acid as set forth in SEQ ID NO:7 or a functional variant at least 98% identical to SEQ ID NO:7,
- the phosphoprotein (P) comprising an amino acid as set forth in SEQ ID NO:
- 8 or a functional variant at least 98% identical to SEQ ID NO:8,
- the large protein (L) comprising an amino acid as set forth in SEQ ID NO:9 or a functional variant at least 98% o identical to SEQ ID NO:9, and
- the matrix protein (M) comprising an amino acid as set forth in SEQ ID NO:
- 10 or a functional variant at least 98% identical to SEQ ID NO:10.
A recombinant rhabdovirus encoding in its RNA genome at least one CD80 extracellular domain Fc-fusion protein or a functional variant thereof, wherein the CD80 extracellular domain Fc-fusion protein comprises the extracellular domain of CD80 and further comprises the Fc domain of an IgG, wherein the RNA genome of the recombinant rhabdovirus comprises or consists of a coding sequence identical or at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.