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US 12,171,787 B2Granted
Methods and compositions for modulating angiogenesis and vasculogenesis
AssigneeSanBio, Inc.
InventorsMonique Dao, Ciara Tate, Casey C. Case
Life of this patent
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Abstract
Disclosed herein are methods and compositions for stimulating angiogenesis, using cells descended from marrow adherent stromal cells that have been transfected with sequences encoding a Notch intracellular domain. Applications of these methods and compositions include treatment of ischemic disorders such as stroke.
Claims10
A method for augmenting angiogenesis in a subject that does not have an ischemic disorder, the method comprising:
- implanting a population of cells into the subject, wherein the cell population is made by a method comprising:
- (a) contacting a culture of mesenchymal stem cells with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein,
- (b) selecting cells that comprise the polynucleotide of step (a), and
- (c) further culturing the selected cells of step (b) in the absence of selection and in the absence of trophic factors;
- wherein the cell population produces higher levels of angiogenin, angiopoietin-2 (ANG-2) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) than does a mesenchymal stem cell population containing the same number of cells.
2 more elements
Dependencies are inferred from the claim text.