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US 12,169,198 B2Granted
Systems and methods for sample analysis
Assignee10x Genomics, Inc.
InventorsAndrew D. Price, Christopher Hindson
Life of this patent
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Abstract
The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.
Claims22
A mixture, comprising:
- a nucleic acid comprising:
- (i) a first moiety, (ii) a nucleic acid sequence, and (ii) a poly-thymine (poly(T)) sequence, a poly-guanine (poly(G)) sequence, or an N-mer sequence;
- a nucleic acid barcode comprising a first strand and a second strand, wherein said first strand comprises a second moiety and a barcode sequence, wherein said second strand comprises (1) a first sequence complementary to said barcode sequence and (2) an overhang comprising a second sequence complementary to said nucleic acid sequence;
- and
- reagents configured to non-enzymatically ligate said first moiety to said second moiety.
2 more elements
Dependencies are inferred from the claim text.