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US 12,168,772 B2Granted
Method of transforming photosynthetic organisms
AssigneeViridos, Inc.
InventorsJohn H. Verruto, Jessica N. Weir
Life of this patent
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Abstract
The invention provides methods of transforming photosynthetic organisms, such as green algae. The methods involve methylating one or more DNA fragments of a DNA construct and transforming the one or more fragments into the photosynthetic organism. The DNA fragments can be the product of a DNA amplification procedure, such as PCR or a PCR-like procedure. In one embodiment the one or more fragments of DNA that comprise a DNA construct are dam methylated prior to being transformed into the photosynthetic organism.
Claims14
A method of transforming a photosynthetic Trebouxiophyte organism comprising:
- contacting at least one DNA fragment with a dam methyltransferase to produce at least one methylated DNA fragment;
- and
- transforming the photosynthetic Trebouxiophyte organism with the at least one methylated DNA fragment.
Dependencies are inferred from the claim text.