Plant regulatory elements and uses thereof
AssigneeMonsanto Technology LLC
InventorsJaishree M. Chittoor, Stanislaw Flasinski
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Abstract
The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules. Also provided are methods of the use of the recombinant DNA molecules and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules and constructs.
Claims32
A recombinant DNA molecule comprising a polynucleotide having a sequence selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to SEQ ID NO:
- 1 or 2 and having promoter activity;
- b) a sequence comprising SEQ ID NO:1 or 2;
- and
- c) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, wherein the fragment has promoter activity;
- wherein said polynucleotide is operably linked to a heterologous transcribable DNA molecule.
A transgenic plant cell comprising a recombinant DNA molecule comprising a polynucleotide selected from the group consisting of:
- a) a sequence with at least 95 percent sequence identity to SEQ ID NO:
- 1 or 2 and having promoter activity;
- b) a sequence comprising SEQ ID NO:1 or 2;
- and
- c) a fragment comprising at least 150 contiguous nucleotides of SEQ ID NO:1 or 2, wherein the fragment has promoter activity;
- wherein said polynucleotide is operably linked to a heterologous transcribable DNA molecule.
A method of expressing a transcribable DNA molecule in root tissue, comprising:
- a) transforming a plant cell with a vector comprising a promoter with at least 95 percent sequence identity to SEQ ID NO:
- 2 and having promoter activity or an active fragment thereof comprising at least 150 contiguous nucleotides of SEQ ID NO:
- 2 operably linked to a heterologous transcribable DNA molecule;
- and
- b) inducing the cell to form a plant.
Dependencies are inferred from the claim text.