Devices, systems, and methods of electronic modulation of polymerase for DNA synthesis
AssigneeGeorgia Tech Research Corporation
InventorsHua Wang, Andrew Hessel
Life of this patent
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Abstract
A method of synthesis of a nucleotide chain, the nucleotide chain including an ordered plurality of nucleotides, the method including: identifying a first nucleotide of the ordered plurality of nucleotides; controlling a polymerase enzyme to assemble the first nucleotide onto the nucleotide chain by electrically modulating an electrode; identifying a subsequent nucleotide in the ordered plurality of nucleotides as a current nucleotide; and controlling the polymerase enzyme to assemble the current nucleotide onto an end of the nucleotide chain by electrically modulating the electrode.
Claims12
A method of de novo nucleotide chain assembly comprising:
- immobilizing a template-independent enzyme to an electrode platform comprising an electrode with a seeded inert surface functionalized with one or more links, the template-independent enzyme immobilized to the electrode platform via one or more of the links;
- controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a first nucleotide of a nucleotide chain by electrically modulating the electrode;
- and
- controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a next nucleotide of the nucleotide chain by electrically modulating the electrode;
- wherein the assembly of the nucleotide chain is a de novo assembly of the nucleotide chain without using primer and without using template enzymes.
A method of de novo deoxyribonucleic acid (DNA) synthesis comprising:
- receiving data indicative of a sequential order of a number (N) of nucleotides forming a nucleic acid sequence to be assembled, the sequential order of the nucleotides forming the nucleic acid sequence including N nucleotides from a first nucleotide, one or more intermediate nucleotides, and to a last nucleotide;
- attaching one or more template-independent DNA polymerases to an electrode platform comprising one or more electrodes, one or more of the electrodes having a seeded inert surface functionalized with one or more bio-links, one or more of the template-independent DNA polymerases attached to the electrode platform via one or more of the bio-links;
- controlling, via direct electrical-field modulation, one or more of the attached template-independent DNA polymerases to assemble the first nucleotide of the nucleic acid sequence by electrically modulating one or more of the electrodes to assemble the first nucleotide of the nucleic acid sequence;
- and
- repeating N−1 times:
- identifying a subsequent nucleotide in the sequential order of nucleotides as a current nucleotide;
- and
- controlling, via direct electrical-field modulation, one or more of the attached template-independent DNA polymerases to assemble the current nucleotide onto an end of the nucleic acid sequence by electrically modulating one or more of the electrodes to assemble the current nucleotide onto the nucleic acid sequence;
- until one or more of the attached template-independent DNA polymerases assembles a complete nucleic acid sequence comprising the sequential order of the nucleotide;
- wherein a subset of two or more electric modulations, from a set containing all of the electric modulations, is selected from a group consisting of a subset of electric modulations each at the same electric modulation, a subset of electric modulations of which at least two are at a different electric modulation one from another, a subset of electric modulations of which each are at a different electric modulation one from the others, and a combination thereof;
- wherein the synthesis of the nucleic acid sequence is a de novo synthesis of the nucleic acid sequence without using primer and without using template DNA polymerases.
Dependencies are inferred from the claim text.