Method for producing a fibrin-based bioartificial, primarily acellular construct, and the construct itself
AssigneeGottfried Wilhelm Leibniz Universitat Hannover
InventorsThomas Aper, Mathias Wilhelmi, Axel Haverich
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Abstract
The invention relates to a method for producing a bioartificial and primarily acellular fibrin-based construct, wherein a mixture of cell-free compositions containing fibrinogen and thrombin is applied to a surface and subsequently pressurised. An additional aspect of the invention is directed to such fibrin-based bioartificial acellular constructs obtained according to the invention, with improved biomechanical properties, as well as to the use of same in the field of implantology, cartilage replacement or tissue replacement.
Claims11
A method for producing a fibrin-based bioartificial and acellular planar construct, comprising:
- provision of a fibrinogen-containing and cell-free composition;
- provision of a thrombin-containing and cell-free composition;
- application of the fibrinogen-containing cell-free and the thrombin-containing cell-free compositions to a mould as an applied layer of a mixture of compositions;
- pressurization of the mixture of compositions applied to the mould at least until the fibrin formation is essentially completed;
- and
- removal of the fibrin-based acellular bioartificial planar construct from the mould,
- wherein pressure is applied in a planar manner to the compositions applied to the mould,
- wherein the pressurization takes place at a relative pressure of at least 1 bar,
- wherein the fibrin-based acellular bioartificial construct has at least a portion with a wall thickness of at least 1 mm, and
- wherein the mould has through holes.
Dependencies are inferred from the claim text.