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US 12,168,028 B2Granted
Methods for obtaining cardiomyogenic precursor cells
AssigneeUniversity of Miami
InventorsJoshua M. Hare, Konstantinos Chatzistergos
Life of this patent
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Abstract
The disclosure provides a method of obtaining a population of cardiomyogenic precursor cells. The method comprises (a) differentiating induced pluripotent stem cells (iPSCs) to iPSC-derived cardiac precursor cells; and (b) isolating cardiac precursor cells expressing mammalian growth hormone-releasing hormone receptor (GHRHR) to obtain a population of iPSC-derived cardiomyogenic precursor cells.
Claims4
A method of obtaining a population of human cardiomyogenic precursor cells, the method comprising:
- (a) differentiating human induced pluripotent stem cells (hiPSCs) in the presence of all of a glycogen synthase kinase 3 (GSK3) inhibitor, a Wnt inhibitor, and recombinant growth hormone releasing hormone or their synthetic analogs to obtain hiPSC-derived human cardiac precursor cells;
- and
- (b) isolating a population of human cardiac precursor cells based on (i) expression of human growth hormone-releasing hormone receptor (GHRHR) using a GHRHR antibody and (ii) no expression of c-Kit to obtain a population consisting essentially of hiPSC-derived human cardiomyogenic precursor cells which express Nkx2.5 and Isl1.
Dependencies are inferred from the claim text.