Non-recombinant human insulin-like growth factor binding protein concentrate

A method of forming a concentrate of a non-recombinant human insulin-like growth factor (IGF) binding protein 3 (nr-IGFBP-3) in a native heterogeneous form in aqueous solution, the method comprising:

• (1) contacting a porous matrix with an aqueous solution, wherein the aqueous solution is human serum or IGF binding protein from the human serum;
• and
• (2) collecting concentrated nr-IGFBP-3 in the native heterogeneous form in aqueous solution from the porous matrix, the collected nr-IGFBP-3 in aqueous solution having a concentration within a range of from about 16 micrograms per milliliter (μg/ml) to about 40 μg/ml;
• and
• wherein the concentrate contains no recombinant IGFBP-3.