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US 12,163,968 B2Granted
Preparation of nucleated RBC (NRBC) analogs for use as reference hematology controls in automated hematology analyzers
AssigneeBio-Rad Laboratories, Inc.
InventorBeena Lee
Life of this patent
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Abstract
The subject invention pertains to compositions of novel analogs of red blood cells that are distinguishable from white blood cells in a hematological instrument and processes for manufacturing such analogs. The processes for creating the compositions include washing, shrinking, and fixing cells at temperatures at or below room temperature.
Claims21
A process for manufacture of red blood cells which are distinguishable from white blood cells for use as a reference control in a blood cell analyzer, the process comprising the steps of:
- a) shrinking and washing red blood cells of a non-human vertebrate by application of a salt solution comprising a denaturing agent, EDTA, potassium cyanide, potassium ferricyanide, sodium nitrate, potassium hydroxide, sodium hydroxide, MOPS, sodium fluoride, mecaptosuccinic acid, sodium omadine, amikacin, tetracycline, gentamycin sulfate, and reagent alcohol to extract cellular fluid from the red blood cells;
- and
- b) applying a quantity of a fixing agent to the red blood cells from step a).
Dependencies are inferred from the claim text.