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US 12,163,918 B1Granted
Compositions and methods for protein electrophoresis
AssigneeProteinSimple
InventorsJames McElroy, Christopher Heger, Annegret Boge, Irina Georgievna Kazakova
Life of this patent
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Abstract
Compositions and methods for protein electrophoresis are described herein. In various embodiments, the compositions and methods of the disclosure provide for reduced protein fragmentation relative to the protein fragmentation caused by alternative compositions and methods.
Claims22
A kit comprising:
- (i) a capillary electrophoresis sodium dodecyl sulfate (CE-SDS) sample loading buffer, comprising:
- a buffered aqueous solution having a pH between 6.0 and 7.7 and configured to load a sample into a capillary for capillary electrophoresis, the buffered aqueous solution including:
- (i-a) an acidic buffer selected from the group consisting of:
- 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-Hydroxy-3-morpholinopropanesulfonic acid (MOPSO), and N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES);
- and
- (ii-a) Bis-Tris Propane (BTP);
- and
- (ii) instructions for use in CE-SDS electrophoresis.
5 more elements
A kit comprising:
- (i) a capillary electrophoresis sodium dodecyl sulfate (CE-SDS) sample loading buffer, comprising:
- a buffered aqueous solution having a pH between 6.0 and 7.7 and configured to load a sample into a capillary for capillary electrophoresis, the buffered aqueous solution including:
- (i-a) an acidic buffer selected from the group consisting of:
- 2-(N-morpholino)ethanesulfonic acid (MES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-Hydroxy-3-morpholinopropanesulfonic acid (MOPSO), and N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES);
- and
- (ii-a) Bis-Tris Propane (BTP);
- and
- (ii) a capillary suitable for CE-SDS.
5 more elements
Dependencies are inferred from the claim text.