Digital mirror device based code-division multiplexed Raman optical mapping system for wide field imaging

A method of mapping a tissue sample, comprising:

• providing a system having a light source configured to emit a beam of light, a scanner, a digital mirror device (DMD) having an orthogonal array of micromirrors, each micromirror selectively controllable to be in an on-state or an off-state, at least one light detector, and an analyzer in communication with the light source, the scanner, the DMD, and the at least one light detector;
• providing a tissue sample inoculated with a material containing pHLIPs conjugated with one or more Raman reporters (RR);
• scanning the DMD with a light beam produced by the light source, the scanning including using the scanner to move the light beam relative to the DMD;
• controlling the DMD to have a subset of micromirrors within the micromirror array in the on-state aligned with the light beam scanning the DMD, and the micromirrors in the micromirror array not in the subset in the off-state, wherein at least some of the micromirrors in the subset of micromirrors in the on-state are configured to direct the light beam to interrogate the tissue sample, wherein the interrogation produces Raman emitted light from the tissue sample;
• assigning one or more location codes to the subset of micromirrors in the on-state indicative of a location of the subset of micromirrors within the micromirror array;
• receiving the Raman emitted light from the tissue sample using the at least one light detector, and producing signals representative of the Raman emitted light;
• correlating the assigned one or more location codes of the subset of micromirrors with the signals representative of the Raman emitted light;
• and
• producing a spatial map of the Raman emitted light using the signals representative of the Raman emitted light and the correlation between the assigned one or more location codes of the subset of micromirrors and the signals.