Information processing apparatus, information processing method, information processing system, and computer program

A non-transitory computer readable storage medium comprising instructions

• that, when executed, cause a processor to at least:
• determine a corrected luminance of pixels of an image of a sample dyed with a fluorescent dye reagent based on a fluorescence fading coefficient, the fluorescence fading coefficient representative of rapidness at which a fluorescence intensity of the fluorescent dye reagent decreases;
• convert the corrected luminance of pixels into at least one of a first number of fluorescent molecules or a second number of antibodies bonded to the fluorescent molecules;
• and
• generate image information based on at least one of the first number of fluorescent molecules or the second number of antibodies.

An apparatus comprising:

• memory;
• a computer-readable program;
• and
• a processor to execute the computer-readable program to at least:
• determine a corrected luminance of pixels of an image of a sample dyed with a fluorescent dye reagent based on a fluorescence fading coefficient, the fluorescence fading coefficient representative of rapidness at which a fluorescence intensity of the fluorescent dye reagent decreases;
• convert the corrected luminance of pixels into at least one of a first number of fluorescent molecules or a second number of antibodies bonded to the fluorescent molecules;
• and
• generate image information based on at least one of the first number of fluorescent molecules or the second number of antibodies.

A system comprising:

• a storage disc or storage device;
• a computer-readable program stored in the storage disc or storage device;
• a processor to execute the computer-readable program to:
• determine a corrected luminance of pixels of an image of a sample dyed with a fluorescent dye reagent based on a fluorescence fading coefficient, the fluorescence fading coefficient representative of rapidness at which a fluorescence intensity of the fluorescent dye reagent decreases;
• convert the corrected luminance of pixels into at least one of a first number of fluorescent molecules or a second number of antibodies bonded to the fluorescent molecules;
• and
• output image information based on at least one of the first number of fluorescent molecules or the second number of antibodies;
• and
• a display to display the image information.