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US 12,163,181 B2Granted
Methods for making nucleotide probes for sequencing and synthesis
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Kun Zhang, Joseph Chou
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Abstract
Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.
Claims13
A method of analyzing a plurality of mRNA samples to obtain allele-specific quantitation at one or more alleles in each mRNA sample, the method comprising the steps of:
- providing one or more probes, wherein a probe of the one or more probes has two regions of homology to target mRNA at the ends of the probe, two primer regions common to other probes of the one or more probes, and a bar code specific for an allele;
- hybridizing the probe to mRNA in a circular manner;
- ligating portions of the probe to one another to produce a closed circular molecule;
- separating the closed circular molecule from the mRNA;
- amplifying the closed circular molecule to produce amplified DNA;
- and
- sequencing the amplified DNA.
4 more elements
Dependencies are inferred from the claim text.