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US 12,163,177 B2Granted
Modified fatty acid biosynthesis with ACP-dependent thiolases
AssigneeRamon Gonzalez
InventorsRamon Gonzalez, James M. Clomburg
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Abstract
Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse β-oxidation cycle.
Claims1
A microorganism, comprising:
- a) an overexpressed acetyl-CoA:
- ACP transacylase encoded by E. coli fabD or Streptomyces collinus fadA;
- b) an overexpressed ACP-dependent thiolase encoded by E. coli atoB, E. coli yqeF, E. coli fadA, E. coli fadl, Streptomyces collinus fadA, Ralstonia eutropha bktB, Pseudomonas sp. Strain B13 catF, E. coli paaf, Pseudomonas putida pcaF, Rhodococcus opacus pcaF, Streptomyces sp. pcaF, Ralstonia eutropha phaA, Clostridium acetobutylicum thlA, or Clostridium acetobutylicum thlB,
- c) an overexpressed 3-oxoacyl- reductase encoded by E. coli fabG;
- d) an overexpressed 3-hydroxyacyl- dehydratase encoded by E. coli fabA, or E. coli fabZ;
- e) an enoyl-[ACP] reductase encoded by E. coli fabI, Enterococcus faecalis fabK, Bacillus subtilis fabL, or Vibrio cholerae fabV;
- and
- f) an overexpressed ACP-cleaving thioesterase encoded by E. coli tesA, Cuphea palustris fatB1, Cuphea viscosissima fatB3, Ulmus americana fatB1, Cocos nucifera fatB2, Elaeis guineensis PTE, Clostridium perfringens CPF 2954, Umbellularia californica fatB1, Bacteroides thetaiotaomicron bTE, Bacteroides fragilis tes4, or Marvinbryantia formatexigens BRYFOR_06758.
5 more elements
Dependencies are inferred from the claim text.