US 12,163,171 B2 · Method for carrying out a polymerase chain reaction and device for carrying out the method

Abstract

A method for amplifying nucleic acids by a polymerase chain reaction in a reaction volume heated using electrical energy. In at least one of the passages of the amplification cycle of the polymerase chain reaction, the ratio of the electrical energy used in the denaturation step to heat the reaction volume to the size of the reaction volume is less than 20 Joule per milliliter. Further shown is a method of amplifying nucleic acids in a reaction volume by using a device that includes a reaction vessel and a heating means with at least one heating element in contact with the reaction volume where at least one heating element is conjugated to oligonucleotides. Also shown is a device for the amplification of nucleic acids in a reaction volume including a reaction vessel for receiving the reaction volume and a heating means consisting of at least one heating element contacting the reaction volume.

Claims12

Dependent claims

A device for amplifying nucleic acids in a reaction volume comprising:

a reaction vessel to receive the reaction volume,
at least one heating element to heat the reaction volume using electrical energy, the at least one heating element comprising a continuous wire at least partially suspended within the reaction vessel, the continuous wire to contact and to be at least partially surrounded by the reaction volume, and
an electrical circuit configured to transfer the electrical energy into the device via the continuous wire, the electrical energy delivered as electrical pulses applied to the continuous wire and configured to cause heat pulses in the reaction volume,
wherein the heat pulses cause a localized heating-up zone within the reaction volume, and
wherein the device is configured such that electrical power consumption of the device during a polymerase chain reaction does not exceed 50 Watt at any point in time.

2 more elements

A device for amplifying nucleic acids in a reaction volume comprising:

a reaction vessel to receive the reaction volume,
at least one heating element that runs through and is disposed in the reaction vessel to heat the reaction volume using electrical energy, the at least one heating element comprising a resistive element suspended within the reaction vessel, the at least one heating element to contact and to be at least partially surrounded by the reaction volume, and
an electrical circuit configured to transfer the electrical energy into the device via electrical pulses applied to the at least one heating element configured to cause heat pulses in the reaction volume, wherein the heat pulses are configured to cause a localized heating-up zone within the reaction volume and wherein the device is configured such that a ratio between electrical power consumption of the device during a polymerase chain reaction and a capacity of the reaction vessel does not exceed 1 Watt per millilitre at any point in time.

Dependencies are inferred from the claim text.

Method for carrying out a polymerase chain reaction and device for carrying out the method

Life of this patent

Method for carrying out a polymerase chain reaction and device for carrying out the method

Life of this patent

Abstract

Claims12