Engineered primate cystine/cysteine degrading enzymes for therapeutic uses
AssigneeBoard of Regents, The University of Texas System
InventorsEverett Stone, Wei-Cheng Lu, Christos Karamitros
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Abstract
Methods and compositions related to the engineering of a protein with L-cyst(e)ine degrading enzyme activity are described. For example, disclosed are modified cystathionine-γ-lyases comprising one or more amino acid substitutions and capable of degrading L-cyst(e)ine. Furthermore, compositions and methods are provided for the treatment of cystinuria using the disclosed modified enzymes or nucleic acids encoding said enzymes.
Claims34
An isolated, modified primate cystathionine-γ-lyase (CGL) enzyme comprising the following substitutions relative to a native human CGL amino acid sequence (SEQ ID NO:
- 1), wherein the modified enzyme has both cystinase and cysteinase activity, said substitutions being
- alanine at position 193, glycine at position 311, valine at position 339, and serine at position 353.
Dependencies are inferred from the claim text.