Plant cells and plants modified to increase resistance to necrotrophs or drought and methods of selecting and using the same
AssigneeWisconsin Alumni Research Foundation
InventorsAshish Ranjan, Mehdi Kabbage, Damon Lee Smith
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Abstract
The invention generally relates to plant cells and plants modified to increase resistance to necrotrophs or drought and methods of selecting and using the same. More specifically, the invention relates in part to plant cells and/or plants modified to eliminate or reduce as compared to control plants cell the NADPH oxidase activity or expression of certain respiratory burst oxidase homolog (RBOH) proteins and methods of selecting for and using the same.
Claims17
A plant cell transformed with a first expression cassette which comprises a nucleic acid molecule operably linked to a heterologous promoter and encoding at least one first polynucleotide that targets at least one endogenous respiratory burst oxidase gene in said plant cell to reduce or eliminate expression of at least one endogenous respiratory burst oxidase protein encoded by said at least one endogenous respiratory burst oxidase gene, wherein said at least one endogenous respiratory burst oxidase protein has at least 97% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1, and wherein overexpression of said first polynucleotide in said plant cell increases resistance to a necrotroph and increases drought tolerance as compared to a control plant cell of the same species lacking said first expression cassette, and wherein the plant cell is selected from the group consisting of a soybean plant cell, a common bean plant cell and a leguminous plant cell.
A method of generating a transgenic plant comprising:
- (i) transforming plant cells with a first expression cassette which comprises a nucleic acid molecule operably linked to a heterologous promoter and encoding at least one first polynucleotide that targets at least one endogenous respiratory burst oxidase gene in said transgenic plant cells to reduce or eliminate expression of at least one endogenous respiratory burst oxidase protein encoded by said at least one respiratory burst oxidase gene, wherein said at least one endogenous respiratory burst oxidase protein has at least 97% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 1, and wherein said plant cells are selected from the group consisting of soybean plant cells, common bean plant cells and leguminous plant cells;
- (ii) obtaining transgenic plants from said transformed plant cells of step (i);
- and (iii) selecting a transgenic plant from the transgenic plants of step (ii) that expresses said first polynucleotide in said selected transgenic plant and exhibits increased resistance to a necrotroph and increased drought tolerance as compared to a control plant of the same plant species lacking said first expression cassette and grown under identical growth conditions.
Dependencies are inferred from the claim text.