Closure for fluid-tight sealing of a vessel filled with a substance, container with a vessel and a closure as well as method for filling a container with a substance

Closure for fluid-tight closing of a vessel filled with a substance, the closure comprising a fluid-tight pressure-transmission element and the closure comprising a base body to which the pressure-transmission element can be attached in a fluid-tight manner, wherein the pressure-transmission element is deflectable by pressurisation without incurring damage, for biological inactivation of microorganisms present in the substance, wherein the closure is configured in such a way that the pressure-transmission element, when the vessel is closed with the closure, is held clamped between the vessel and the base body;

• wherein the base body comprises a plurality of tamper-evident elements, wherein the base body is configured to be attached to the vessel without damage to the plurality of tamper-evident elements and to be separated from the vessel with damage to at least one of the plurality of tamper-evident elements;
• wherein the base body has a longitudinal body axis along which the directions proximal and distal are defined, wherein proximal, in the intended use, points towards an interior of the vessel and distal opposite thereto, wherein at least two of the plurality of tamper-evident elements are arranged at different positions along said longitudinal body axis.