Methods and compositions for purification or isolation of microvesicles and exosomes
AssigneeExopharm Limited
InventorsGregor Lichtfuss, Jim Palmer, Ian Dixon
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Abstract
The invention provides methods and compositions for the isolation or extraction of exosomes or microvesicles. The methods and compositions may involve providing a device including exosome-binding ligands in the form of anionic or electron-rich groups arranged on a substrate surface. The methods and compositions may involve providing a device including a substrate having a surface, with a plurality of polymers attached to the substrate and exosome-binding ligands in the form of anionic or electron-rich groups arranged on the plurality of polymers.
Claims20
A method for obtaining an isolate or composition of exosomes or microvesicles including:
- providing a liquid that includes exosomes or microvesicles;
- providing a device for binding to exosomes or microvesicles, the device including:
- a substrate having a surface;
- a plurality of polymers attached to the substrate;
- one or more exosome binding ligands in the form of anionic groups at pH 4-8 arranged on the polymers;
- the polymers extending from the substrate surface to position an exosome binding ligand at a location that is spaced apart from the substrate surface for binding to exosomes or microvesicles, thereby enabling exosomes or microvesicles to bind to the device;
- contacting the liquid with the device in conditions enabling the binding of exosomes or microvesicles to the one or more exosome-binding ligands;
- and
- separating the device from the liquid;
- thereby obtaining an isolate or composition of exosomes or microvesicles.
Dependencies are inferred from the claim text.