Methods of use of islet cells
AssigneeCedars-Sinai Medical Center
InventorsClive Svendsen, Dhruv Sareen
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Abstract
Type 2 diabetes (T2D) is a clinical syndrome caused by insufficient insulin secretion for insulin requirements. described herein are compositions and methods for microphysiological MPS models of disease (MODs) for diabetes. These platforms allow one to compare the effect of chronic β-cell stimulation in the presence and absence of patient specific immune cells in IPSC-derived islets from each group. Additionally, one can reproduce the T2D β-cell phenotype, using islets-on-chips will also be exposed to gluco-lipotoxicity. Likewise, skeletal muscle-on-chips are exposed to patient specific activated immune cells, variable motor neuron innervation and lipids characteristic of T2D.
Claims15
A method of generating pancreatic progenitor cells, comprising:
- culturing induced pluripotent stem cells (iPSCs) in the presence of Activin A and CHIR99021 for about 3 days to generate definitive endoderm;
- culturing definitive endoderm in the presence of FGF10, Noggin, and CHIR 99021 for about 2 days to generate gut tube endothelium;
- and
- culturing gut tube endothelium in the presence of FGF10, Noggin, Sant1, and retinoic acid for about 4 days to generate pancreatic progenitor cells.
Dependencies are inferred from the claim text.