Production method for culture supernatant preparation
AssigneeLaboratory of Cell Applied Technologies, Co
InventorsHajime Inoue, Chiharu Fujita
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Abstract
To provide a culture supernatant preparation which has excellent biocompatibility and contains a large quantity of specific genes or proteins. A method for producing the culture supernatant preparation including: a first culturing step of culturing cells to a confluent state using a first medium; a second culturing step of culturing the cells using a second medium that is different from the first medium after the first culturing step; and a culture supernatant preparation obtaining step of obtaining the culture supernatant preparation including the second medium after the second culturing step, the second medium including a calcium ion and a buffering agent.
Claims11
A method for producing a culture supernatant preparation, said method comprising:
- a first culturing step of culturing a cell to a confluent state using a first medium;
- a second culturing step of culturing the cell using a second medium that is different from the first medium after the first culturing step;
- and
- a culture supernatant preparation obtaining step of obtaining the culture supernatant preparation including the second medium after the second culturing step,
- wherein the second medium is an infusion liquid for injection or an infusion liquid for intravenous drip infusion.
Dependencies are inferred from the claim text.