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US 12,161,672 B2Granted
Chimeric antigen receptor and methods of use thereof
AssigneeThe Regents of the University of California
InventorsChia-Yung Wu, James Onuffer, Wendell A. Lim
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Abstract
The present disclosure provides a heterodimeric, conditionally active chimeric antigen receptor (CAR), and a nucleic acid comprising a nucleotide sequence encoding the CAR. The present disclosure provides cells genetically modified to produce the CAR. A CAR of the present disclosure can be used in various methods, which are also provided.
Claims18
A method for activating a T lymphocyte, comprising:
- contacting:
- (a) a T lymphocyte expressing a heterodimeric, conditionally active chimeric antigen receptor (CAR) comprising:
- (i) a first polypeptide comprising:
- a first member of a specific binding pair;
- a first member of a dimerization pair;
- and
- a transmembrane domain between the first member of the specific binding pair and the first member of the dimerization pair;
- and
- (ii) a second polypeptide comprising:
- a second member of the dimerization pair;
- and
- an immunoreceptor tyrosine-based activation motif (ITAM);
- with:
- (b) a dimerization agent that dimerizes the first and second members of the dimerization pair;
- and
- (c) a target cell that comprises a cell surface antigen to which the first member of the specific binding pair binds;
- wherein binding of the first polypeptide to the antigen on the surface of the target cell in the presence of the dimerization agent results in signal transduction via the ITAM of the second polypeptide and activation of the T lymphocyte.
14 more elements
Dependencies are inferred from the claim text.