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US 12,161,122 B2Granted
Compositions comprising pesticide precursors and methods of making and use
AssigneeMustGrow Biologics Corp.
InventorJames Robinson
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Abstract
Disclosed is a two-part pesticide formulation and methods of making and using. The pesticide formulation comprises a first part comprising a glucosinolate concentrate, and a second part comprising a plant material comprising myrosinase. The parts may be stored and transported in inactive form, and are activated upon application to a substrate requiring pesticide treatment.
Claims15
A two-part fungicide precursor system comprising
- (a) a first contained part comprising a sinigrin-containing glucosinolate concentrate obtained from a Brassica plant material comprising less than 0.5% of glucosinolate breakdown products, and
- (b) a second separately contained part comprising a Sinapis alba plant material comprising an active myrosinase complex in a concentration sufficient to release an effective amount of allyl isothiocyanate upon mixing of the first and second part in the presence of water,
- and
- wherein the amount of sinigrin in the first part, the amount and activity of the myrosinase complex in the second part, and the relative amounts of the first and second parts are such that, upon mixing of the two parts in the presence of water, a fungicide formulation is obtained in which the myrosinase activity is less than 0.5 of myrosinase enzyme activity units/mg of sinigrin naturally present in Brassica plant material, and more than 12.5 μmol of sinigrin is hydrolyzed per minute per unit of active myrosinase complex.
1 more element
A method of preparing a two-part fungicide precursor system comprising preparing
- (a) a first contained part comprising a sinigrin-containing glucosinolate concentrate obtained from a Brassica plant material comprising less than 0.5% of glucosinolate breakdown products, and
- (b) a second separately contained part comprising a Sinapis alba plant material comprising an active myrosinase complex in a concentration sufficient to release an effective amount of allyl isothiocyanate upon mixing of the first and second component in the presence of water,
- wherein the amount of sinigrin in the first part, the amount and activity of the myrosinase complex in the second part, and the relative amounts of the first and second parts are such that, upon mixing of the two parts in the presence of water, a fungicide formulation is obtained in which the myrosinase activity is less than 0.5 myrosinase enzyme activity units/mg of sinigrin naturally present in Brassica plant material, and more than 12.5 μmol of sinigrin is hydrolyzed per minute per unit of active myrosinase complex.
A method for controlling fungal pests comprising:
- (a) providing a two-part fungicide precursor system, said system comprising:
- (i) a first contained part comprising an aqueous sinigrin-containing glucosinolate concentrate obtained from a Brassica plant material less than 0.5% of glucosinolate breakdown products comprising in excess of 10% (v/v) water;
- and (ii) a second separately contained part comprising a Sinapis alba plant material comprising an active myrosinase complex in a concentration sufficient to release an effective amount of allyl isothiocyanate upon the addition of water to the mixture;
- (b) mixing the first part and second part of the system to obtain a mixture wherein the amount of sinigrin in the first part, the amount and activity of the myrosinase complex in the second part, and the relative amounts of the first and second parts are such that, upon mixing of the two parts, a mixture is obtained in which the myrosinase activity is less than one half of the myrosinase activity per unit mass of sinigrin naturally present in the Brassica plant material;
- (c) adding water to the mixture to obtain a fungicide formulation;
- and
- (d) applying the fungicide formulation to a substrate in need of fungicide treatment, wherein upon mixing of the two parts in step (c) the fungicide formulation is obtained wherein the myrosinase activity is less than 0.5 myrosinase enzyme activity units/mg of sinigrin naturally present in the Brassica plant material, and wherein more than 12.5 μmol of sinigrin is hydrolyzed per minute per unit of active myrosinase complex.
4 more elements
Dependencies are inferred from the claim text.